ExoELISA-ULTRA Complete Kit (CD9 Detection)

Quickly quantify exosomes using CD9 detection with this sensitive ELISA-based assay suitable for most biofluids
  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. SmartSEC, ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Products

Catalog Number Description Size Price Quantity Add to Cart
EXEL-ULTRA-CD9A-1 ExoELISA-ULTRA Complete Kit (CD9 Detection) 96 Reactions $636
- +

Overview

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD9 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours. 

Configured for detection of CD9, a common exosomal marker, ExoELISA-ULTRA CD9 is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with common biofluids. To enable quantitation of exosomes carrying CD9 in your target sample, the kit includes an internal standard calibrated to exosomes from a range of biofluids that have been analyzed by NTA analysis.

One ExoELISA-ULTRA CD9 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. SmartSEC, ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Choose the exosome quantitation method that’s best for your studies

 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet

How It Works

Supporting Data

Supporting Data

The standard curve for ExoELISA-ULTRA CD9 shows robust linearity down to ~2 x 109 exosomes.

Calibration curve and example data for ExoELISA-ULTRA Complete Kit (CD9 Detection)

Figure 1. ExoELISA-ULTRA CD9 standard curve shows robust linearity down to ~2 x 109 exosomes. OD450 nm values of commonly used samples fall well within the standard curve for the assay.

 

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
EXEL-ULTRA-CD9A-1 ExoELISA-ULTRA Complete Kit (CD9 Detection) 96 Reactions $636
- +

Overview

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD9 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours. 

Configured for detection of CD9, a common exosomal marker, ExoELISA-ULTRA CD9 is based on an ultra-sensitive direct capture colorimetric ELISA that is compatible with common biofluids. To enable quantitation of exosomes carrying CD9 in your target sample, the kit includes an internal standard calibrated to exosomes from a range of biofluids that have been analyzed by NTA analysis.

One ExoELISA-ULTRA CD9 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. SmartSEC, ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Choose the exosome quantitation method that’s best for your studies

 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet

How It Works

Supporting Data

Supporting Data

The standard curve for ExoELISA-ULTRA CD9 shows robust linearity down to ~2 x 109 exosomes.

Calibration curve and example data for ExoELISA-ULTRA Complete Kit (CD9 Detection)

Figure 1. ExoELISA-ULTRA CD9 standard curve shows robust linearity down to ~2 x 109 exosomes. OD450 nm values of commonly used samples fall well within the standard curve for the assay.

 

Citations