ExoQuick UltraPure (EV Isolation Kit)
Pure EVs. Higher Yields. Ultra Performance
Why choose between yield and purity?
With SBI’s ExoQuick® UltraPure, you get both. This next-generation hybrid EV isolation kit combines gentle precipitation with advanced spin-column technology to deliver maximum yields of pure, apoB-free, intact EVs in just 20 minutes - no ultracentrifuge required. Compared to size-exclusion chromatography (SEC) and other polymer-based methods, ExoQuick® UltraPure (or EQ UltraPure) achieves lower albumin carry-over and higher purity, making it the smarter choice for reliable EV isolation. In figures and tables, the product may be referred to as EQ UltraPure (its catalog reference) for space efficiency. Both names refer to the same product and can be used interchangeably depending on context.
- Dual-Action Innovation – integrates SBI’s proprietary ExoQuick® gentle precipitation with advanced mixed-mode affinity spin columns for unmatched purity and yield
- Small Sample Compatibility – efficient EV isolation from as little as 100 µL to 250 µL of serum or plasma
- ExoQuick® UltraPure: Unmatched Quality, Backed by Data - the only kit delivering high yield, high purity, concentrated EVs with superior protein, total RNA, and cleaner samples
ExoQuick® UltraPure vs. SEC and Ultracentrifugation
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EQ UltraPure | ExoQuick® UltraPure EV Isolation Kit for Serum and plasma* |
10 rxn | $399 |
|
||||
Kit contents
| Item | Volume/ Quantity | Storage Temperature |
|---|---|---|
| ExoQuick® | 1 ml, Sterile |
+4 °C to +30 °C
|
| UltraPure purification column | 10 columns | |
| Column Buffer | 25 ml | |
| Collection tubes | 10 | |
| 2 ml Eppendorf tubes | 10 |
Overview
ExoQuick® UltraPure kit is engineered for excellence in extracellular vesicle (EV) isolation field, delivering high recovery rates and outstanding purity to meet the demands of cutting-edge EV research. This advanced kit incorporates SBI’s trusted ExoQuick® EV isolation reagent with innovative mixed-mode chromatography affinity columns for a dual-action workflow that ensures:
- All EVs are captured by precipitation using ExoQuick® reagents
- Maximum purity through mixed-mode chromatography that integrates size exclusion, affinity interactions, and lipoprotein depletion
Key benefits of the ExoQuick® UltraPure kit include:
- Maximized EV Recovery — Enhanced formulation yields significantly higher EV counts compared to conventional reagents
- Exceptional Purity — Advanced mixed-mode chromatography minimizes protein carry-over and lipoprotein contamination
- Consistent & Reliable Performance — As opposed to ultracentifugation and regular SEC methods, ExoQuick® UltraPure delivers highly reproducible results across experiments
- User-Friendly Workflow — No ultracentrifugation or fractionation; Just add ExoQuick® solution to sample, mix, spin, transfer to column, brief incubation, quick spin, done
Optimized for processing small-volume samples such as serum or plasma, the EQ UltraPure kit generates EVs ready for a range of downstream applications, including:
- EV nucleic acid and protein analysis/profiling
- EV quantification and characterization
- EV engineering
- In vivo/ex vivo EV delivery
- EV biomarker discovery
- EV-based therapeutic development
With its simplicity, reliability, and superior performance, the EQ UltraPure is a powerful, game-changing tool for advancing EV research and driving breakthroughs in diagnostics, therapeutics, and beyond.
References
How It Works
Figure 1. ExoQuick® UltraPure Workflow
Supporting Data
Supporting Data
ExoQuick® UltraPure delivers superior EV purity with higher yields
Figure 2. ExoQuick® UltraPure Delivers Higher Yields of Pure EVs. Western blot of serum-derived EVs shows EQ UltraPure retains key EV markers (CD9, CD81, TSG101) while reducing albumin contamination compared to ExoQuick®, ExoQuick® Ultra, and SmartSEC™ Single.
ExoQuick® UltraPure Sets a New Benchmark in EV Isolation Performance
Figure 3. ExoQuick® UltraPure Outperforms Traditional EV Isolation Methods EVs were isolated from serum using EQ UltraPure, SEC, and ultracentrifugation. EQ UltraPure shows the strongest EV marker signals (CD9, TSG101), the lowest albumin contamination, and delivers the highest EV yields by protein and fNTA compared to SEC and ultracentrifugation.
- (A) Western blot analysis. Each lane was loaded with an equal amount of EV protein.
- (B) Total EV protein yield per mL of serum. EV protein concentration was measured using a NanoDrop spectrophotometer.
- (C) Fluorescent nanoparticle tracking analysis (fNTA) demonstrates the superior EV yields obtained with EQ UltraPure compared to SEC and ultracentrifugation.
- (D–E) Comparative analysis of EV isolation methods based on yield per input serum volume (D, per mL) and per input EV protein (E, per mg).
EQ UltraPure = Cleaner Intact EVs, Fewer Lipoproteins
Figure 4. ExoQuick® UltraPure Enables Efficient Lipoprotein Removal ELISA analysis shows EQ UltraPure removes ~96% of ApoB from serum, yielding the lowest lipoprotein contamination. In contrast, SEC and ultracentrifugation preparations contain 3–4× higher ApoB levels.
- (A) ApoB lipoprotein levels measured by ELISA.
- (B) EQ UltraPure removes ~ 96% of ApoB from serum.
High-Quality EV RNA, Every Time.
Figure 5. EV RNA Quality Assessment by BioAnalyzer EV RNA isolated with EQ UltraPure and the EVery EV RNA Kit (Cat# Every100B-1) shows high quality by Agilent Bioanalyzer electropherogram and gel analysis.
FAQs
Documentation
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EQ UltraPure | ExoQuick® UltraPure EV Isolation Kit for Serum and plasma* |
10 rxn | $399 |
|
||||
Kit contents
| Item | Volume/ Quantity | Storage Temperature |
|---|---|---|
| ExoQuick® | 1 ml, Sterile |
+4 °C to +30 °C
|
| UltraPure purification column | 10 columns | |
| Column Buffer | 25 ml | |
| Collection tubes | 10 | |
| 2 ml Eppendorf tubes | 10 |
Overview
ExoQuick® UltraPure kit is engineered for excellence in extracellular vesicle (EV) isolation field, delivering high recovery rates and outstanding purity to meet the demands of cutting-edge EV research. This advanced kit incorporates SBI’s trusted ExoQuick® EV isolation reagent with innovative mixed-mode chromatography affinity columns for a dual-action workflow that ensures:
- All EVs are captured by precipitation using ExoQuick® reagents
- Maximum purity through mixed-mode chromatography that integrates size exclusion, affinity interactions, and lipoprotein depletion
Key benefits of the ExoQuick® UltraPure kit include:
- Maximized EV Recovery — Enhanced formulation yields significantly higher EV counts compared to conventional reagents
- Exceptional Purity — Advanced mixed-mode chromatography minimizes protein carry-over and lipoprotein contamination
- Consistent & Reliable Performance — As opposed to ultracentifugation and regular SEC methods, ExoQuick® UltraPure delivers highly reproducible results across experiments
- User-Friendly Workflow — No ultracentrifugation or fractionation; Just add ExoQuick® solution to sample, mix, spin, transfer to column, brief incubation, quick spin, done
Optimized for processing small-volume samples such as serum or plasma, the EQ UltraPure kit generates EVs ready for a range of downstream applications, including:
- EV nucleic acid and protein analysis/profiling
- EV quantification and characterization
- EV engineering
- In vivo/ex vivo EV delivery
- EV biomarker discovery
- EV-based therapeutic development
With its simplicity, reliability, and superior performance, the EQ UltraPure is a powerful, game-changing tool for advancing EV research and driving breakthroughs in diagnostics, therapeutics, and beyond.
References
How It Works
Figure 1. ExoQuick® UltraPure Workflow
Supporting Data
Supporting Data
ExoQuick® UltraPure delivers superior EV purity with higher yields
Figure 2. ExoQuick® UltraPure Delivers Higher Yields of Pure EVs. Western blot of serum-derived EVs shows EQ UltraPure retains key EV markers (CD9, CD81, TSG101) while reducing albumin contamination compared to ExoQuick®, ExoQuick® Ultra, and SmartSEC™ Single.
ExoQuick® UltraPure Sets a New Benchmark in EV Isolation Performance
Figure 3. ExoQuick® UltraPure Outperforms Traditional EV Isolation Methods EVs were isolated from serum using EQ UltraPure, SEC, and ultracentrifugation. EQ UltraPure shows the strongest EV marker signals (CD9, TSG101), the lowest albumin contamination, and delivers the highest EV yields by protein and fNTA compared to SEC and ultracentrifugation.
- (A) Western blot analysis. Each lane was loaded with an equal amount of EV protein.
- (B) Total EV protein yield per mL of serum. EV protein concentration was measured using a NanoDrop spectrophotometer.
- (C) Fluorescent nanoparticle tracking analysis (fNTA) demonstrates the superior EV yields obtained with EQ UltraPure compared to SEC and ultracentrifugation.
- (D–E) Comparative analysis of EV isolation methods based on yield per input serum volume (D, per mL) and per input EV protein (E, per mg).
EQ UltraPure = Cleaner Intact EVs, Fewer Lipoproteins
Figure 4. ExoQuick® UltraPure Enables Efficient Lipoprotein Removal ELISA analysis shows EQ UltraPure removes ~96% of ApoB from serum, yielding the lowest lipoprotein contamination. In contrast, SEC and ultracentrifugation preparations contain 3–4× higher ApoB levels.
- (A) ApoB lipoprotein levels measured by ELISA.
- (B) EQ UltraPure removes ~ 96% of ApoB from serum.
High-Quality EV RNA, Every Time.
Figure 5. EV RNA Quality Assessment by BioAnalyzer EV RNA isolated with EQ UltraPure and the EVery EV RNA Kit (Cat# Every100B-1) shows high quality by Agilent Bioanalyzer electropherogram and gel analysis.