Cold Fusion Cloning Kit with Competent Cells

Fast, easy, and efficient, the phosphatase-free, ligation-free Cold Fusion Cloning Kit will take you to transformation-ready DNA in 20 minutes and a single step
  • Fast—get transformation-ready DNA in as little as twenty minutes
  • Easy—a phosphatase-free and ligation-free system that eliminates the need for specific restriction enzymes
  • Efficient—typically achieve >90% positive clones
  • Versatile—add any insert into any site in any vector using manual or automated workflows

Products

Catalog Number Description Size Price Quantity Add to Cart
MC010B-1 Cold Fusion Cloning Kit with Competent Cells 10 Reactions $241.00
- +
Contact Us
MC096B-1 Cold Fusion Cloning Kit with Competent Cells - 96-well format 96 Reactions $1,781.00
- +
Contact Us
MC100B-1 Cold Fusion Cloning Kit with Competent Cells 20 Reactions $440.00
- +
Contact Us
MC101B-1 Cold Fusion Cloning Kit with Competent Cells 50 Reactions $1,013.00
- +
Contact Us

Overview

Overview

Quick and easy ligation-free cloning Fast, easy, and efficient, SBI’s Cold Fusion Cloning is an excellent choice for any cloning project. Whether you're assembling multiple fragments of DNA or simply adding an insert or gBlock® to a vector, the Cold Fusion Cloning Kit will take you to transformation-ready DNA in a single step—just incubate your DNA fragment(s) with linearized vector for five minutes at room temperature and ten minutes on ice. A typical cloning reaction will deliver a successful cloning rate of more than 90%.
  • Fast—get transformation-ready DNA in as little as twenty minutes
  • Easy—a phosphatase-free and ligation-free system that eliminates the need for specific restriction enzymes
  • Efficient—typically achieve >90% positive clones
  • Versatile—add any insert into any site in any vector using manual or automated workflows
“I have designed and made hundreds and hundreds of clones using the traditional and not so traditional methods since I started doing research. I must say the Cold Fusion kit makes it way too easy to design a vector or new construct—it’s a really good product.” ~Hidevaldo B. Machado, PhD., UCLA

How It Works

How It Works

Streamlining vector construction with Cold Fusion Cloning

Cold Fusion Cloning is a quick and easy way to place an insert—or multiple inserts simultaneously—into a linearized vector. The Cold Fusion Master mix contains a proprietary blend of enzymes that prepares DNA fragments ends for sequence-directed alignment. Homologous DNA ends are efficiently fused together and produce vector clones with great accuracy.

The four-step process is as follows (see workflow illustration below):

  1. Linearize your vector and amplify your insert using primers with at least 15 bp of homology to the ends of your linearized vector.
  2. Mix your PCR-amplified inserts with the linearized vector.
  3. Incubate for 5 minutes at room temperature and then 10 minutes on ice to fuse the insert to the linearized vector.
  4. Transform competent cells with the fusion mix. A typical Cold Fusion Cloning reaction delivers 90% positive clones.

How to use SBI’s easy and efficient Cold Fusion Cloning Kit

Supporting Data

Supporting Data

See the results of a better way to clone

Cold Fusion Cloning can clone 3

Figure 1. Cold Fusion Cloning can clone 3′, 5′, and blunt-end fragments efficiently.

Simultaneously insert multiple fragments into a vector in a defined order

Figure 2. Simultaneously insert multiple fragments into a vector in a defined order. Using Cold Fusion technology, the team at SBI built a 4-in-1 construct to generate iPSCs in only three days.

Resources

Citations

  • Gentile, A, et al. (2021) The EMT transcription factor Snai1 maintains myocardial wall integrity by repressing intermediate filament gene expression. eLife. 1970 Jan 1; 10. PM ID: 34152269
  • Schmidt, C, Perkovic, M & Schnierle, BS. (2021) Development of a Sensitive Detection Method for Alphaviruses and Its Use as a Virus Neutralization Assay. Viruses. 1970 Jan 1; 13(7). PM ID: 34206519
  • Lv, M & Liu, Q. (2021) JMJD2C triggers the growth of multiple myeloma cells via activation of β‑catenin. Oncology reports. 1970 Jan 1;. PM ID: 33469678
  • Bejarano, F, et al. (2021) A comprehensive in vivo screen for anti-apoptotic miRNAs indicates broad capacities for oncogenic synergy. Developmental Biology. 1970 Jan 1;. Link: Developmental Biology
  • Wang, W, et al. (2021) miR-181c regulates MCL1 and cell survival in GATA2 deficient cells. Journal of leukocyte biology. 1970 Jan 1;. PM ID: 34270823
  • Bishop, D, et al. (2020) CAR T cell generation by piggyBac transposition from linear doggybone DNA vectors requires transposon DNA flanking regions.. Molecular Therapy - Methods & Clinical Development. 1970 Jan 1;. Link: Molecular Therapy - Methods & Clinical Development
  • Mo, Y, et al. (2020) Down-regulation of microRNA-34c-5p alleviates neuropathic pain via the SIRT1/STAT3 signaling pathway in rat models of chronic constriction injury of sciatic nerve. J. Neurochem.. 1970 Jan 1;. PM ID: 32126145
  • Moore, KB, et al. (2020) Localized calcium signaling and the control of coupling at Cx36 gap junctions. eNeuro. 1970 Jan 1;. PM ID: 32179580
  • Ou, M, et al. (2020) Emerging roles of let‑7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation. Int J Mol Med. 1970 Jan 1;. Link: Int J Mol Med
  • Papadogiannis, V. (2020) The genetics of cranial sensory ganglia development and evolution. Thesis. 1970 Jan 1;. Link: Thesis
  • Lagunes, L. (2020) Scaffolding, Multisite Phosphorylation and Other Aspects of Regulation in Signal Transduction. Thesis. 1970 Jan 1;. Link: Thesis
  • Carlantoni, C, et al. (2020) Tie1 regulates zebrafish cardiac morphogenesis through tolloid-like 1 expression. Dev. Biol.. 1970 Jan 1;. PM ID: 32971120
  • Fahim, A, et al. (2020) Characterization of natural antisense transcripts arisen from the locus encoding Toxoplasma gondii ubiquitin-like protease. Molecular and Biochemical Parasitology. 1970 Jan 1;:111334. Link: Molecular and Biochemical Parasitology
  • Papdogiannis, V, et al. (2020) Hmx gene conservation identifies the evolutionary origin of vertebrate cranial ganglia. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Santhanam, A, et al. (2020) A Zebrafish Model of Retinitis Pigmentosa Shows Continuous Degeneration and Regeneration of Rod Photoreceptors. Cells. 1970 Jan 1; 9(10). PM ID: 33036185
  • Kathiriya, IS, et al. (2020) Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease. Developmental cell. 1970 Jan 1;. PM ID: 33321106
  • Apaydin, DC, et al. (2020) Early-Life Stress Regulates Cardiac Development through an IL-4-Glucocorticoid Signaling Balance. Cell reports. 1970 Jan 1; 33(7):108404. PM ID: 33207196
  • Vergarajauregui, S, et al. (2020) AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9. eLife. 1970 Jan 1; 9. PM ID: 33295871
  • Shao, Z, et al. (2019) Epstein-Barr Virus BALF0 and BALF1 Modulate Autophagy. Viruses. 1970 Jan 1; 11(12). PM ID: 31783609
  • Liu, D, et al. (2019) Long noncoding RNA ADAMTS9‐AS2 suppresses the progression of esophageal cancer by mediating CDH3 promoter methylation. Molecular carcinogenesis. 1970 Jan 1; 59(1):32-44. PM ID: 31621118

Products

Catalog Number Description Size Price Quantity Add to Cart
MC010B-1 Cold Fusion Cloning Kit with Competent Cells 10 Reactions $241.00
- +
Contact Us
MC096B-1 Cold Fusion Cloning Kit with Competent Cells - 96-well format 96 Reactions $1,781.00
- +
Contact Us
MC100B-1 Cold Fusion Cloning Kit with Competent Cells 20 Reactions $440.00
- +
Contact Us
MC101B-1 Cold Fusion Cloning Kit with Competent Cells 50 Reactions $1,013.00
- +
Contact Us

Overview

Overview

Quick and easy ligation-free cloning Fast, easy, and efficient, SBI’s Cold Fusion Cloning is an excellent choice for any cloning project. Whether you're assembling multiple fragments of DNA or simply adding an insert or gBlock® to a vector, the Cold Fusion Cloning Kit will take you to transformation-ready DNA in a single step—just incubate your DNA fragment(s) with linearized vector for five minutes at room temperature and ten minutes on ice. A typical cloning reaction will deliver a successful cloning rate of more than 90%.
  • Fast—get transformation-ready DNA in as little as twenty minutes
  • Easy—a phosphatase-free and ligation-free system that eliminates the need for specific restriction enzymes
  • Efficient—typically achieve >90% positive clones
  • Versatile—add any insert into any site in any vector using manual or automated workflows
“I have designed and made hundreds and hundreds of clones using the traditional and not so traditional methods since I started doing research. I must say the Cold Fusion kit makes it way too easy to design a vector or new construct—it’s a really good product.” ~Hidevaldo B. Machado, PhD., UCLA

How It Works

How It Works

Streamlining vector construction with Cold Fusion Cloning

Cold Fusion Cloning is a quick and easy way to place an insert—or multiple inserts simultaneously—into a linearized vector. The Cold Fusion Master mix contains a proprietary blend of enzymes that prepares DNA fragments ends for sequence-directed alignment. Homologous DNA ends are efficiently fused together and produce vector clones with great accuracy.

The four-step process is as follows (see workflow illustration below):

  1. Linearize your vector and amplify your insert using primers with at least 15 bp of homology to the ends of your linearized vector.
  2. Mix your PCR-amplified inserts with the linearized vector.
  3. Incubate for 5 minutes at room temperature and then 10 minutes on ice to fuse the insert to the linearized vector.
  4. Transform competent cells with the fusion mix. A typical Cold Fusion Cloning reaction delivers 90% positive clones.

How to use SBI’s easy and efficient Cold Fusion Cloning Kit

Supporting Data

Supporting Data

See the results of a better way to clone

Cold Fusion Cloning can clone 3

Figure 1. Cold Fusion Cloning can clone 3′, 5′, and blunt-end fragments efficiently.

Simultaneously insert multiple fragments into a vector in a defined order

Figure 2. Simultaneously insert multiple fragments into a vector in a defined order. Using Cold Fusion technology, the team at SBI built a 4-in-1 construct to generate iPSCs in only three days.

Citations

  • Gentile, A, et al. (2021) The EMT transcription factor Snai1 maintains myocardial wall integrity by repressing intermediate filament gene expression. eLife. 1970 Jan 1; 10. PM ID: 34152269
  • Schmidt, C, Perkovic, M & Schnierle, BS. (2021) Development of a Sensitive Detection Method for Alphaviruses and Its Use as a Virus Neutralization Assay. Viruses. 1970 Jan 1; 13(7). PM ID: 34206519
  • Lv, M & Liu, Q. (2021) JMJD2C triggers the growth of multiple myeloma cells via activation of β‑catenin. Oncology reports. 1970 Jan 1;. PM ID: 33469678
  • Bejarano, F, et al. (2021) A comprehensive in vivo screen for anti-apoptotic miRNAs indicates broad capacities for oncogenic synergy. Developmental Biology. 1970 Jan 1;. Link: Developmental Biology
  • Wang, W, et al. (2021) miR-181c regulates MCL1 and cell survival in GATA2 deficient cells. Journal of leukocyte biology. 1970 Jan 1;. PM ID: 34270823
  • Bishop, D, et al. (2020) CAR T cell generation by piggyBac transposition from linear doggybone DNA vectors requires transposon DNA flanking regions.. Molecular Therapy - Methods & Clinical Development. 1970 Jan 1;. Link: Molecular Therapy - Methods & Clinical Development
  • Mo, Y, et al. (2020) Down-regulation of microRNA-34c-5p alleviates neuropathic pain via the SIRT1/STAT3 signaling pathway in rat models of chronic constriction injury of sciatic nerve. J. Neurochem.. 1970 Jan 1;. PM ID: 32126145
  • Moore, KB, et al. (2020) Localized calcium signaling and the control of coupling at Cx36 gap junctions. eNeuro. 1970 Jan 1;. PM ID: 32179580
  • Ou, M, et al. (2020) Emerging roles of let‑7d in attenuating pulmonary arterial hypertension via suppression of pulmonary artery endothelial cell autophagy and endothelin synthesis through ATG16L1 downregulation. Int J Mol Med. 1970 Jan 1;. Link: Int J Mol Med
  • Papadogiannis, V. (2020) The genetics of cranial sensory ganglia development and evolution. Thesis. 1970 Jan 1;. Link: Thesis
  • Lagunes, L. (2020) Scaffolding, Multisite Phosphorylation and Other Aspects of Regulation in Signal Transduction. Thesis. 1970 Jan 1;. Link: Thesis
  • Carlantoni, C, et al. (2020) Tie1 regulates zebrafish cardiac morphogenesis through tolloid-like 1 expression. Dev. Biol.. 1970 Jan 1;. PM ID: 32971120
  • Fahim, A, et al. (2020) Characterization of natural antisense transcripts arisen from the locus encoding Toxoplasma gondii ubiquitin-like protease. Molecular and Biochemical Parasitology. 1970 Jan 1;:111334. Link: Molecular and Biochemical Parasitology
  • Papdogiannis, V, et al. (2020) Hmx gene conservation identifies the evolutionary origin of vertebrate cranial ganglia. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Santhanam, A, et al. (2020) A Zebrafish Model of Retinitis Pigmentosa Shows Continuous Degeneration and Regeneration of Rod Photoreceptors. Cells. 1970 Jan 1; 9(10). PM ID: 33036185
  • Kathiriya, IS, et al. (2020) Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease. Developmental cell. 1970 Jan 1;. PM ID: 33321106
  • Apaydin, DC, et al. (2020) Early-Life Stress Regulates Cardiac Development through an IL-4-Glucocorticoid Signaling Balance. Cell reports. 1970 Jan 1; 33(7):108404. PM ID: 33207196
  • Vergarajauregui, S, et al. (2020) AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9. eLife. 1970 Jan 1; 9. PM ID: 33295871
  • Shao, Z, et al. (2019) Epstein-Barr Virus BALF0 and BALF1 Modulate Autophagy. Viruses. 1970 Jan 1; 11(12). PM ID: 31783609
  • Liu, D, et al. (2019) Long noncoding RNA ADAMTS9‐AS2 suppresses the progression of esophageal cancer by mediating CDH3 promoter methylation. Molecular carcinogenesis. 1970 Jan 1; 59(1):32-44. PM ID: 31621118