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XPack™ Technology

Engineer exosome protein cargo

Exosome Protein Loading

  • Pack reporter proteins to track cargo
  • Make biotherapeutic exosomes
  • Generate bioluminescent exosomes
  • Create stable cell lines loading desired protein cargo

Online Resources

Technical References and Product Citations

Peterson MF, Otoc N, Sethi JK, Gupta A, Antes TJ. Integrated systems for exosome investigation. Methods. 2015 Apr 24. pii: S1046-2023(15)00161-9.

Gyorgy B, Hung ME, Breakefield XO, Leonard JN. Therapeutic applications of extracellular vesicles: clinical promise and open questions. Annu Rev Pharmacol Toxicol. 2015;55:439-64.

Chu IM, Hengst L, Slingerland JM. The Cdk inhibitor p27 in human cancer: prognostic potential and relevance to anticancer therapy". Nat. Rev. Cancer 8 (4): 253-67.

van der Meel R, Fens MH, Vader P, van Solinge WW, Eniola-Adefeso O, Schiffelers RM. Extracellular vesicles as drug delivery systems: lessons from the liposome field. J Control Release. 2014 Dec 10;195:72-85.

Coleman BM, Hill AF. Extracellular vesicles - Their role in the packaging and spread of misfolded proteins associated with neurodegenerative diseases. Semin Cell Dev Biol. 2015 Feb 20. pii: S1084-9521(15)00034-8.

Yao Y, Wei W, Sun J, Chen L, Deng X, Ma L, Hao S. Proteomic analysis of exosomes derived from human lymphoma cells. Eur J Med Res. 2015 Jan 29;20(1):8.

Yang J, Wei F, Schafer C, Wong DT. Detection of tumor cell-specific mRNA and protein in exosome-like microvesicles from blood and saliva. PLoS One. 2014 Nov 14;9 (11):e110641.

Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J. Quantitative proteomic analysis of exosome protein content changes induced by hepatitis B virus in Huh-7 cells using SILAC labeling and LC-MS/MS. J Proteome Res. 2014 Dec 5;13(12):5391-402.

Revenfeld AL, Baek R, Nielsen MH, Stensballe A, Varming K, Jorgensen M. Diagnostic and prognostic potential of extracellular vesicles in peripheral blood. Clin Ther. 2014 Jun 1;36(6):830-46.

Zhang L, Wrana JL. The emerging role of exosomes in Wnt secretion and transport. Curr Opin Genet Dev. 2014 Aug;27:14-9.

Drake RR, Kislinger T. The proteomics of prostate cancer exosomes. Expert Rev Proteomics. 2014 Apr;11(2):167-77.

Camacho L, Guerrero P, Marchetti D. MicroRNA and protein profiling of brain metastasis competent cell-derived exosomes. PLoS One. 2013 Sep 16;8(9):e73790.

Raimondo F, Morosi L, Corbetta S, Chinello C, Brambilla P, Della Mina P, Villa A, Albo G, Battaglia C, Bosari S, Magni F, Pitto M. Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes. J Extracell Vesicles. 2012 Apr 16;1.

de Jong OG, Verhaar MC, Chen Y, Vader P, Gremmels H, Posthuma G, Schiffelers RM, Gucek M, van Balkom BW. Differential protein profiling of renal cell carcinoma urinary exosomes. Mol Biosyst. 2013 Jun;9(6):1220-33.

Yang JM, Gould SJ. Protein targeting to exosomes/microvesicles by plasma membrane anchors. Biochem Soc Trans. 2013 Feb 1;41(1):277-82.

Shen B, Wu N, Yang JM, Gould SJ. The cis-acting signals that target proteins to exosomes and microvesicles. J Biol Chem. 2011 Apr 22;286(16):14383-95.