XPack-Luciferase Exosome Packing

  

The XPack-Luciferase fusion protein (~62 kD) is packaged as exosome cargo and can be delivered to target cells. HEK-293 cells were first transfected with the XPack-Luc lentivector plasmid (cat# XPAK532PA-1) encoding the XPack-Luciferase fusion protein. The secreted exosomes were collected from the media after 48 hours using ExoQuick-TC®. Luciferase levels of isolated exosomes from untransfected control cells and XPack-Luc transfected cells were quantitated using standard luminescence assays (right panel).

XPack Delivers Luciferase to Target Cells

XPack-Luciferase exosomes (250 ug) were then added to approximately 400,000 HEK-293 recipient cells 6-well plate culture dish. Luciferase activity within these recipient cells were measured after 24 hours and data are shown below. Controls included cells not treated with exosomes (Untreated cells) or cells incubated with exosomes from untransfected HEK-293 cells (Cells + HEK exosomes). The image inset is from imaging live HEK-293 cells treated with either Control or XPack-Luc exosomes for 24 hours. Approximately 400,000 HEK-293 cells were incubated with 150ng/ml D-Luciferin substrate for 2 minutes at ambient temperature prior to signal imaging.