Support
Contact SBI for technical support.
Locate vector sequences and read FAQs.
System Biosciences (SBI)
2438 Embarcadero Way
Palo Alto, CA 94303
Direct: 650-968-2200
Toll Free: 888-266-5066
Fax: 650-968-2277
Lists of mRNA targets in shRNA Libraries
Human 50K Library List of Target Genes (.zip) »
Mouse 40K Library List of Target Genes (.zip) »
Human Kinase Library List of Target Genes (PDF) »
Human Phosphatase Library List of Target Genes (PDF) »
Human Apoptosis Library List of Target Genes (PDF) »
Frequently Asked Questions (FAQs)
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Will SBI’s VSV-G pseudotyped lentivirus particles infect my cells of interest? |
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Most cell-types and tissues tested are capable of successful lentiviral transduction. To view a profile of cell lines evaluated, download this file (PDF) ». |
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What are the Biosafety concerns when handling SBI’s lentiviruses? |
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The basic requirements are a working laminar flow hood and biosafety level 2 procedures. Follow this link to view the recommendations by the Center for Disease Control (CDC website information). |
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How are the shRNA libraries quality controlled?? |
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SBI's QC of our shRNA libraries is to transform bacterial cells with the pooled plasmid library and then select and sequence more than 20 randomly selected clones. We typically obtain less than 10% concatemeric inserts and greater than 90% shRNA inserts. |
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How many shRNAs are designed per messenger RNA target? |
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The libraries are designed in such a way so that there are 3 to 5 shRNA for each target gene. So there is a lot of redundancy built into the library, thus a 50% unique shRNA representation will yield reliable results. |