Support
Contact SBI for technical support.
Locate vector sequences and read FAQs.
System Biosciences (SBI)
2438 Embarcadero Way
Palo Alto, CA 94303
Direct: 650-968-2200
Toll Free: 888-266-5066
Fax: 650-968-2277
ExoQuick Frequently Asked Questions (FAQs)
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How do I decide which ExoQuick product to start with? ExoQuick or ExoQuick-TC?? | ||||||||||||||||||||
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ExoQuick should be used with biofluids that have a high concentration of exosomes like serum, plasma, or ascites fluid. ExoQuick-TC should be used with biofluids that are dilute and contain a lower concentration of exosomes like tissue culture (cell culture) media, urine, and CSF. | ||||||||||||||||||||
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Can I freeze my biofluid before using ExoQuick or ExoQuick-TC to isolate the exosomes? | ||||||||||||||||||||
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Yes! Biofluids can be frozen following their isolation/preparation and should be thawed on ice. Once thawed, you can proceed with the regular ExoQuick or ExoQuick-TC protocol. | ||||||||||||||||||||
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What are the typical yields of exosome, protein and RNAs from various biological fluids? | ||||||||||||||||||||
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See the values in the Table below. These are average yields.
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When I used ExoQuick, a large insoluble pellet was obtained and now it is difficult to work with. What should I do? | ||||||||||||||||||||
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Typically this happens if starting with plasma or serum. With plasma, there are a lot of fibrins and fibrinogens that also get precipitated with ExoQuick. We recommend adding the Thromboplastin D protocol before adding the ExoQuick, per the steps in the user manual. For serum, this typically occurs if you have incubated the ExoQuick with the serum for too long, or if the centrifugation step was too long. See the troubleshooting table below.
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What should I resuspend the precipitated exosomes with? | ||||||||||||||||||||
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This depends on what you want to do with the exosomes. Follow the guidelines here:
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