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PhiC31 Integrase Reprogramming

Nonviral induction of pluripotent stem cells

Make nonviral iPSCs

  • All-in-one iPSC vector
  • Single copy integration
  • Removable with FLP
  • Highly efficient

The phiC31 Integrase System


Nonviral technology for transgenesis
The phiC31 integrase is a site-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two ~34 base pair sequences termed attachment sites (att), one found in the phage and the other in the bacterial host. This serine integrase has been show to function efficiently in many different cell types including mammalian cells. In the presence of phiC31 integrase, an attB containing donor plasmid can be unidirectional integrated into a target genome through recombination at sites with sequence similarity to the native attP site. These sites are termed pseudo-attP sites. phiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable.