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iPSC Reprogramming Factors

For induction of pluripotent stem cells

Study pluripotency

  • Oct4
  • Nanog and Sox2
  • KLF4 or Lin28
  • c-Myc

Induced Pluripotency Stem Cells (iPSCs) using Retroviruses

Human foreskin fibroblasts (HFFs) or Mouse embryonic fibroblasts (MEFs) are transduced with high-titer iPSC "clean" retrovirus pools to induce pluripotency. The result is high quality iPS cells with no fluorescent markers or drug selection required. The entire process takes up to 6 weeks. The iPS Cell lines are then expanded for later certification for pluripotency markers SSEA-4/1, Nanog and for AP activity.

Retrovirus reprogramming