iPSC Reprogramming Factors
For induction of pluripotent stem cells
- Nanog and Sox2
- KLF4 or Lin28
Induced Pluripotency Stem Cells (iPSCs) using Retroviruses
Human foreskin fibroblasts (HFFs) or Mouse embryonic fibroblasts (MEFs) are transduced with high-titer iPSC "clean" retrovirus pools to induce pluripotency. The result is high quality iPS cells with no fluorescent markers or drug selection required. The entire process takes up to 6 weeks. The iPS Cell lines are then expanded for later certification for pluripotency markers SSEA-4/1, Nanog and for AP activity.