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Episomal Reprogramming

Non-viral, non-integrating plasmid
system for clean iPSCs

Make Transgene-free iPSCs

  • Complete mixture with OSK, L-Myc
       shRNA-p53, miR302/367 and GFP
  • Simple protocol for reliable reprogramming
  • Reprogram elderly fibroblast cells efficiently
  • Compatible with feeder or feeder-free conditions

The Episomal iPSC Reprogramming System Works Fast

Generate Transgene-free iPS Cell Lines Rapidly
Transfect target cells only once with the Episomal Reprogramming plasmid mixture (Catalog# SC900A-1). Transfection efficiency can be monitored using the co-expressed GFP marker. Visible iPSC colony formation can be observed in as little as two weeks and is complete in 25 days with 70% more iPSC colonies generated compared to standard OSKM methods. The data below in the left panel show the GFP signals in Human adult primary dermal fibroblasts one day after electroporation. The progression and derivation of validated iPS cells under feeder-free conditions with SBI's PSGro® Human ESC/iPSC Growth Medium for feeder-free conditions (Catalog# SC500M-1) plus SBI's PSGen® Reprogramming Supplement (Catalog# SC551M-1) can be viewed in the right hand panels.

Episomal reprogramming

The Episomal oriP/EBNA-1 reprogramming system also works on difficult-to-reprogram source cells

Older patient dermal fibroblast samples can often be difficult to reprogram. The Episomal oriP/EBNA-1 Reprogramming plasmid mix also works on these difficult-to-reprogram source cells. The panel to the left shows successful iPSC generation using a passage 8 human dermal fibroblasts from a 60 year-old patient sample, whereas standard Yamanaka OSKM factors (right panel) failed to induce any iPSC formation.

Episomal reprogramming