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Antibody and AP Stem Cell Staining Kits

Detect pluripotency protein markers and AP activity in stem cells

Confirm pluripotency in stem cells

  • Immunostain antibodies for pluripotency
  • Oct4, Nanog, SSEA-3, Sox2, TRA-1-60, TRA-1-81
  • Confirm AP activity in stem cells
  • Robust enzymatic readout
  • Choose AP-Red or AP-Blue

Monitor Pluripotency Protein Markers and Alkaline Phosphatase Activity in Stem Cells

Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have distinct pluripotency protein markers like the transcription factors Oct4 and Nanog and show elevated levels of Alkaline Phosphatase (AP) activity. SBI offers affinity purified and validated antibodies to detect these human pluripotent stem cell markers Oct4, Nanog, SSEA-3 and TRA-1-60. The antibodies are available individually, or can be purchased as a complete iPS verification kit that comes with all four antibodies plus an alkaline phosphatase staining kit. Alkaline phosphatase (AP) is a universal pluripotent marker for all types of pluripotent stem cells including embryonic stem cells, embryonic germ cells, and induced pluripotent stem cells. The pluripotent status of stem cells can be characterized by a high level of AP expression, along with the expression of multiple pluripotency markers including the transcription factors Nanog, Oct4, Sox2, stage-specific embryonic antigens, SSEA-1, -3, -4, and tumor related antigens, TRA-1-60, TRA-1-81.

AP is the Universal Pluripotency Marker