Track Neural Differentiation using qPCR
Multi-lineage qPCR array
- Collection of qPCR assays for neural lineages
- Quantitate mRNAs, microRNAs & lncRNAs
- Monitor neural stem cell differentiation
Track Neural Differentiation Using qPCR
During embryonic development, pluripotent stem cells differentiate into three germ layers: ectoderm, mesoderm and endoderm. These germ layers differentiate into multipotent stem cells and progenitor cells, which progress into terminally differentiated cells. For neural differentiation, cells from ectoderm differentiate into neural stem cells, neural progenitor cells and eventually terminally differentiated neurons, oligodendrocytes and astrocytes. This developmental process is accompanied by highly regulated changes in gene expression and expression of non-coding RNAs, such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). For example, neural stem cells and precursor cells are characterized by the expression of Nestin, Sox2 and Pax6; dopaminergic neurons are characterized by the expression of tyrosine hydroxylase (TH); and astrocytes are characterized by the expression of GFAP. Analysis of specific cellular markers during the differentiation process is important for identifying intermediately or terminally differentiated neural cells.
SBI’s Neural Lineage qPCR Profiler provides a comprehensive system for reliable and robust characterization of different stages of human neural differentiation and different populations of terminally differentiated neural cells. The system can simultaneously quantitate fold-change differences for 95 separate genes, microRNAs and lncRNAs between two or more experimental RNA samples. All of the genes were carefully curated from published literature with regards to potential roles in neural differentiation or cell-specific expression for different types of neural cells.