XStamp™-NCAM Lentivector for Targeting Exosomes to neurons
- Display NCAM sequences on exosome surfaces
- Target neurons
- Create stable XStamp-NCAM cell lines
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| XSTP721PA-1 | XStamp NCAM brain targeting lentivector - uses NCAM to target exosomes to the brain | 10 µg | $787 |
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Overview
Overview
Putting exosomes to work: Target exosomes to neurons Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to neurons with XStamp-NCAM. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-NCAM Lentivector fuses NCAM sequences to the XStamp tag, ensuring that exosomes display the NCAM homophilic binding domain on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-NCAM Lentivector will now be targeted to NCAM-expressing cells.- Display NCAM sequences on exosome surfaces
- Target neurons
- Create stable XStamp-NCAM cell lines
SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:
| Cat.# | Specificity | Targeting Ligand Used |
|---|---|---|
| XSTP710PA-1 | Clone-your-own | You decide |
| XSTP720PA-1 | GI tract cells | Motilin |
| XSTP721PA-1 | Neural cells | NCAM |
| XSTP722PA-1 | Brain cells | BHP1 |
| XSTP723PA-1 | Cancer cells | GE11 |
| XSTP724PA-1 | Breast cancer cells | HER2 |
| XSTP725PA-1 | Antigen-presenting cells | CD40L/CD154 |
| XSTP726PA-1 | Immune cells | IL-2 |
How It Works
How It Works
Engineering exosomes with specific surface proteins
XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.
Supporting Data
Supporting Data
See XStamp targeting in action
Figure 1. Both XStamp-NCAM and XStamp-BHP1 can target exosomes to neurons. METHODS: The XStamp-NCAM Lentivector (Cat.# XSTP721PA-1) and the XStamp-BHP1 Lentivector (Cat.# XSTP722PA-1), were transfected separately into mouse MSCs along with SBI’s XPack-GFP construct (Cat.# XPAK530PA-1), which packages GFP into the interior of exosomes to enable fluorescent tracking. Exosomes were isolated after 48 hours, and added to Neuro2a neuroblastoma cells in culture. The neurons were monitored for GFP delivery using phase and fluorescence microscopy after a 24-hour incubation.
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Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| XSTP721PA-1 | XStamp NCAM brain targeting lentivector - uses NCAM to target exosomes to the brain | 10 µg | $787 |
|
||||
Overview
Overview
Putting exosomes to work: Target exosomes to neurons Use exosomes to deliver protein, RNA, DNA, or small molecule cargo to neurons with XStamp-NCAM. The patented XStamp technology is an optimized, exosome surface display system that efficiently places protein sequences on exosomal surfaces. The XStamp-NCAM Lentivector fuses NCAM sequences to the XStamp tag, ensuring that exosomes display the NCAM homophilic binding domain on their outer surface. Exosomes made from cells transfected or transduced with the XStamp-NCAM Lentivector will now be targeted to NCAM-expressing cells.- Display NCAM sequences on exosome surfaces
- Target neurons
- Create stable XStamp-NCAM cell lines
SBI also offers a selection of pre-built targeting XStamp Lentivectors, as well as a clone-your-own XStamp Cloning and Expression Vector:
| Cat.# | Specificity | Targeting Ligand Used |
|---|---|---|
| XSTP710PA-1 | Clone-your-own | You decide |
| XSTP720PA-1 | GI tract cells | Motilin |
| XSTP721PA-1 | Neural cells | NCAM |
| XSTP722PA-1 | Brain cells | BHP1 |
| XSTP723PA-1 | Cancer cells | GE11 |
| XSTP724PA-1 | Breast cancer cells | HER2 |
| XSTP725PA-1 | Antigen-presenting cells | CD40L/CD154 |
| XSTP726PA-1 | Immune cells | IL-2 |
How It Works
How It Works
Engineering exosomes with specific surface proteins
XStamp is based upon a C-terminal fusion of the C1C2 domain from the protein MFG-E8, which is targeted to the exosome surface. Protein sequences that are fused to the XStamp tag will efficiently display the protein ligand fusion on the surfaces of secreted exosomes.
Supporting Data
Supporting Data
See XStamp targeting in action
Figure 1. Both XStamp-NCAM and XStamp-BHP1 can target exosomes to neurons. METHODS: The XStamp-NCAM Lentivector (Cat.# XSTP721PA-1) and the XStamp-BHP1 Lentivector (Cat.# XSTP722PA-1), were transfected separately into mouse MSCs along with SBI’s XPack-GFP construct (Cat.# XPAK530PA-1), which packages GFP into the interior of exosomes to enable fluorescent tracking. Exosomes were isolated after 48 hours, and added to Neuro2a neuroblastoma cells in culture. The neurons were monitored for GFP delivery using phase and fluorescence microscopy after a 24-hour incubation.