XMIRXpress lentivector miRNA-1-3p with Xmotif
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
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XMIRXP-1 | XMIRXpress lentivector miRNA-1-3p with Xmotif | 10 µg | $637 |
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XMIRXP-122 | XMIRXpress lentivector miRNA-122-5p with Xmotif | 10 µg | $637 |
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XMIRXP-124a | XMIRXpress lentivector miRNA-124a-3p with Xmotif | 10 µg | $637 |
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XMIRXP-125b | XMIRXpress lentivector miRNA-125b-5p with Xmotif | 10 µg | $637 |
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XMIRXP-155 | XMIRXpress lentivector miRNA-155-5p with Xmotif | 10 µg | $637 |
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XMIRXP-200c | XMIRXpress lentivector miRNA-200c-3p with Xmotif | 10 µg | $637 |
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XMIRXP-205 | XMIRXpress lentivector miRNA-205-5p with Xmotif | 10 µg | $637 |
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XMIRXP-21 | XMIRXpress lentivector miRNA-21-5p with Xmotif | 10 µg | $637 |
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XMIRXP-219 | XMIRXpress lentivector miRNA-219a-5p with Xmotif | 10 µg | $637 |
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XMIRXP-224 | XMIRXpress lentivector miRNA-224-5p with Xmotif | 10 µg | $637 |
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XMIRXP-29a | XMIRXpress lentivector miRNA-29a-3p with Xmotif | 10 µg | $637 |
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XMIRXP-29b | XMIRXpress lentivector miRNA-29b-3p with Xmotif | 10 µg | $637 |
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XMIRXP-34a | XMIRXpress lentivector miRNA-34a-5p with Xmotif | 10 µg | $637 |
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XMIRXP-486 | XMIRXpress lentivector miRNA-miR-486-5p with Xmotif | 10 µg | $637 |
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Overview
Overview
Putting exosomes to work: Creating stable cell lines that produce miRNA-delivering exosomes
Expanding on our powerful XMIR and AXMIR technologies, SBI developed the XMIRXpress Lentivector System, which enables the creation of stable cell lines that produce exosomes carrying a specific miRNA. Just as with XMIR and AXMIR, you can take advantage of normal cellular processes to package a specific miRNA into exosomes, and then use those exosomes to deliver that miRNA to target cells. Choose from one of our pre-built, ready-to-transfect XMIRXpress Lentivectors (see list below). If you don’t see the miRNA you’re interested in, simply contact us at tech@systembio.com and we’ll design and synthesize the custom XMIR for you, or build your own using the XMIRXpress Cloning Lentivector.
All XMIRXpress Lentivectors feature an EF1a-GFP-Puro selection cassette and a downstream H1 promoter expressing the XMIR + XMotif cassette.
- Flexible—turn the cell line of your choice into a factory that produces miRNA-packed exosomes
- Powerful—great for a range of applications, including:
- Modulating expression of miRNA-targets in recipient cells
- Developing exosome-delivered miRNA-based therapies
How It Works
How It Works
miRNA packaging into exosomes with XMIRXpress
Like XMIR and AXMIR, XMIRXpress packaging relies on an RNA sequence tag—the XMotif (identified and optimized by SBI)—that targets small RNAs to exosomes for packaging. By expressing an miRNA-XMotif cassette from an H1 promoter, you can generate miRNAs that are packaged into exosomes. After creating a stable cell line with your XMIRXpress Lentivector, you can continuously isolate exosomes containing your miRNA.
Supporting Data
Supporting Data
The XMotif in XMIRXpress directs miRNAs to exosomes
Exosomes isolated from cells transfected with the XMIRXpress Lentivector miR-29b only contain packaged XMIRXpress-miR-29b miRNA if the lentivector contains a functional XMotif.
FAQs
Resources
Related Products
Citations
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
XMIRXP-1 | XMIRXpress lentivector miRNA-1-3p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-122 | XMIRXpress lentivector miRNA-122-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-124a | XMIRXpress lentivector miRNA-124a-3p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-125b | XMIRXpress lentivector miRNA-125b-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-155 | XMIRXpress lentivector miRNA-155-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-200c | XMIRXpress lentivector miRNA-200c-3p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-205 | XMIRXpress lentivector miRNA-205-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-21 | XMIRXpress lentivector miRNA-21-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-219 | XMIRXpress lentivector miRNA-219a-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-224 | XMIRXpress lentivector miRNA-224-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-29a | XMIRXpress lentivector miRNA-29a-3p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-29b | XMIRXpress lentivector miRNA-29b-3p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-34a | XMIRXpress lentivector miRNA-34a-5p with Xmotif | 10 µg | $637 |
|
||||
XMIRXP-486 | XMIRXpress lentivector miRNA-miR-486-5p with Xmotif | 10 µg | $637 |
|
Overview
Overview
Putting exosomes to work: Creating stable cell lines that produce miRNA-delivering exosomes
Expanding on our powerful XMIR and AXMIR technologies, SBI developed the XMIRXpress Lentivector System, which enables the creation of stable cell lines that produce exosomes carrying a specific miRNA. Just as with XMIR and AXMIR, you can take advantage of normal cellular processes to package a specific miRNA into exosomes, and then use those exosomes to deliver that miRNA to target cells. Choose from one of our pre-built, ready-to-transfect XMIRXpress Lentivectors (see list below). If you don’t see the miRNA you’re interested in, simply contact us at tech@systembio.com and we’ll design and synthesize the custom XMIR for you, or build your own using the XMIRXpress Cloning Lentivector.
All XMIRXpress Lentivectors feature an EF1a-GFP-Puro selection cassette and a downstream H1 promoter expressing the XMIR + XMotif cassette.
- Flexible—turn the cell line of your choice into a factory that produces miRNA-packed exosomes
- Powerful—great for a range of applications, including:
- Modulating expression of miRNA-targets in recipient cells
- Developing exosome-delivered miRNA-based therapies
How It Works
How It Works
miRNA packaging into exosomes with XMIRXpress
Like XMIR and AXMIR, XMIRXpress packaging relies on an RNA sequence tag—the XMotif (identified and optimized by SBI)—that targets small RNAs to exosomes for packaging. By expressing an miRNA-XMotif cassette from an H1 promoter, you can generate miRNAs that are packaged into exosomes. After creating a stable cell line with your XMIRXpress Lentivector, you can continuously isolate exosomes containing your miRNA.
Supporting Data
Supporting Data
The XMotif in XMIRXpress directs miRNAs to exosomes
Exosomes isolated from cells transfected with the XMIRXpress Lentivector miR-29b only contain packaged XMIRXpress-miR-29b miRNA if the lentivector contains a functional XMotif.