Cytosol Cyto-Tracer™, pCT-Cyto-RFP (CMV)
Study cellular processes and physiology with this Cyto-Tracer that expresses un-tagged RFP from the CMV promoter - untagged RFP localizes in the cytosol
- Stable lentivector-based system
- Great for creating stable reporter cell lines
- Ideal for co-localization studies
- Monitor cellular dynamics and functional studies in real time
- Label cells with RFP for a broad range of studies
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CYTO119-PA-1 | pCT-Cyto-RFP (pCMV, Cytosol, Untagged) | 10 µg | $643 |
|
||||
| CYTO119-VA-1 | pCT-Cyto-RFP (pCMV, Cytosol, Untagged; pre-packaged virus) | >2x10^6 IFUs | $741 |
|
||||
Overview
Overview
Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™
Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-RFP (CMV), expresses an untagged RFP, which naturally localizes to the cytosol. RFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.- Stable lentivector-based system
- Great for creating stable reporter cell lines
- Ideal for co-localization studies
- Monitor cellular dynamics and functional studies in real time
- Label cells with RFP for a broad range of studies
| Promoter (Cat.#) | Cyto-Tracer | Target Location | Peptide Tag |
|---|---|---|---|
| CMV (CYTO100-PA-1/CYTO100-VA-1) MSCV (CYTO100-PB-1/CYTO100-VB-1) | pCT-Mem-GFP | Plasma Membrane | Neuromodulin |
| CMV (CYTO101-PA-1/CYTO101-VA-1) MSCV (CYTO101-PB-1/CYTO101-VB-1) | pCT-InnerMem-GFP | Inner Leaflet of Membrane | Farnesylation signal |
| CMV (CYTO102-PA-1/CYTO102-VA-1) MSCV (CYTO102-PB-1/CYTO102-VB-1) | pCT-Mito-GFP | Mitochondria | Cytochrome C oxidase VIII |
| CMV (CYTO103-PA-1/CYTO103-VA-1) | pCT-ER-GFP | Endoplasmic Reticulum | ER targeting consensus |
| CMV (CYTO104-PA-1/CYTO104-VA-1) | pCT-Golgi-GFP | Golgi Apparatus | Galactosyltrasferase |
| CMV (CYTO105-PA-1/CYTO105-VA-1) | pCT-Secretory-GFP | ER-Golgi Vesicles | Secretory consensus peptide |
| CMV (CYTO106-PA-1/CYTO106-VA-1) | pCT-Lyso-GFP | Lysosome | Cathepsin B |
| CMV (CYTO107-PA-1/CYTO107-VA-1) | pCT-Endo-GFP | Endosome | RhoB |
| CMV (CYTO107R-PA-1/CYTO107R-VA-1) | pCT-Endo-RFP | Endosome | RhoB |
| CMV (CYTO108-PA-1/CYTO108-VA-1) MSCV (CYTO108-PB-1/CYTO108-VB-1) | pCT-Pero-GFP | Peroxisome | Peroxisomal consensus signal |
| CMV (CYTO109-PA-1/CYTO109-VA-1) | pCT-Autophago-GFP | Autophagosome | LC3 |
| CMV (CYTO109R-PA-1/CYTO109R-VA-1) | pCT-Autophago-RFP | Autophagosome | LC3 |
| CMV (CYTO120-PA-1/CYTO120-VA-1) | pCT-CD63-GFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO120R-PA-1/CYTO120R-VA-1) | pCT-CD63-RFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO122-PA-1/CYTO122-VA-1) | pCT-CD9-GFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO123-PA-1/CYTO123-VA-1) | pCT-CD9-RFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO124-PA-1/CYTO124-VA-1) | pCT-CD81-GFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO125-PA-1/CYTO125-VA-1) | pCT-CD81-RFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO110-PA-1/CYTO110-VA-1) MSCV (CYTO110-PB-1/CYTO110-VB-1) | pCT-Actin-GFP | Cytoskeleton | β-actin |
| CMV (CYTO111-PA-1/CYTO111-VA-1) MSCV (CYTO111-PB-1/CYTO111-VB-1) | pCT-Tublin-GFP | Cytoskeleton | α-tublin |
| CMV (CYTO111R-PA-1/CYTO111R-VA-1) | pCT-Tublin-RFP | Cytoskeleton | α-tublin |
| CMV (CYTO112-PA-1/CYTO112-VA-1) | pCT-MAP4-GFP | Microtubule | Microtubule associated protein 4 (MAP4) |
| CMV (CYTO113-PA-1/CYTO113-VA-1) MSCV (CYTO113-PB-1/CYTO113-VB-1) | pCT-H2B-GFP | Nucleus | H2B |
| CMV (CYTO114-PA-1/CYTO114-VA-1) | pCT-Apoptosis-Luc | Caspase 3/7 activation | Cyclic inactive luciferase |
| CMV (CYTO115-PA-1/CYTO115-VA-1) | pCT-GFP-BAX | Cytosol to Mitochondria | BAX |
| CMV (CYTO116-PA-1/CYTO116-VA-1) | pCT- Catenin-GFP | Cytosol to Nucleus/Cell Membrane | β-Catenin activation |
| CMV (CYTO117-PA-1/CYTO117-VA-1) | pCT-CMV-PSD95-GFP | Dendrite Membranes | PSD-95 |
| CMV (CYTO118-PA-1/CYTO118-VA-1) MSCV (CYTO118-PB-1/CYTO118-VB-1) | pCT-Cyto-GFP | Cytosolic Compartment | GFP-Untagged |
| CMV (CYTO119-PA-1/CYTO119-VA-1) | pCT-Cyto-RFP | Cytosolic Compartment | RFP-Untagged |
| MSCV (CYTO121-PB-1/CYTO121-VB-1) | pCT-Cyto-GFpLuc | GFP and Luciferase Control | GFP and Luciferase (Untagged) |
How It Works
How It Works
Visualizing the cell with Cyto-Tracers
SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.
Supporting Data
Supporting Data
See some of our Cyto-Tracers in action
FAQs
Resources
Related Products
Citations
-
Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019; 10(1):1194. PM ID: 30886143
-
Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017; 35(7):1747-1759. PM ID: 28376567
-
Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017; 38(6):592-603. PM ID: 28402394
-
Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. 2017;. Link: Thesis
-
Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017; 292(6):2470-2484. PM ID: 28003368
-
Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016; 37(1):313-21. PM ID: 26211004
-
Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015; 6:7506. PM ID: 26103923
-
Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. 2015;. Link: Thesis
-
Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. 2015;. Link: Thesis
-
VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. 2015;. Link: Thesis
-
Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014; 289(47):32526-37. PM ID: 25261472
-
Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014; 5:5513. PM ID: 25423885
-
Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014; 5:5266. PM ID: 25366663
Cytosol Cyto-Tracer™, pCT-Cyto-RFP (CMV)
$643.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CYTO119-PA-1 | pCT-Cyto-RFP (pCMV, Cytosol, Untagged) | 10 µg | $643 |
|
||||
| CYTO119-VA-1 | pCT-Cyto-RFP (pCMV, Cytosol, Untagged; pre-packaged virus) | >2x10^6 IFUs | $741 |
|
||||
Overview
Overview
Easily study the dynamics of cellular processes with SBI’s Cyto-Tracers™
Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-RFP (CMV), expresses an untagged RFP, which naturally localizes to the cytosol. RFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.- Stable lentivector-based system
- Great for creating stable reporter cell lines
- Ideal for co-localization studies
- Monitor cellular dynamics and functional studies in real time
- Label cells with RFP for a broad range of studies
| Promoter (Cat.#) | Cyto-Tracer | Target Location | Peptide Tag |
|---|---|---|---|
| CMV (CYTO100-PA-1/CYTO100-VA-1) MSCV (CYTO100-PB-1/CYTO100-VB-1) | pCT-Mem-GFP | Plasma Membrane | Neuromodulin |
| CMV (CYTO101-PA-1/CYTO101-VA-1) MSCV (CYTO101-PB-1/CYTO101-VB-1) | pCT-InnerMem-GFP | Inner Leaflet of Membrane | Farnesylation signal |
| CMV (CYTO102-PA-1/CYTO102-VA-1) MSCV (CYTO102-PB-1/CYTO102-VB-1) | pCT-Mito-GFP | Mitochondria | Cytochrome C oxidase VIII |
| CMV (CYTO103-PA-1/CYTO103-VA-1) | pCT-ER-GFP | Endoplasmic Reticulum | ER targeting consensus |
| CMV (CYTO104-PA-1/CYTO104-VA-1) | pCT-Golgi-GFP | Golgi Apparatus | Galactosyltrasferase |
| CMV (CYTO105-PA-1/CYTO105-VA-1) | pCT-Secretory-GFP | ER-Golgi Vesicles | Secretory consensus peptide |
| CMV (CYTO106-PA-1/CYTO106-VA-1) | pCT-Lyso-GFP | Lysosome | Cathepsin B |
| CMV (CYTO107-PA-1/CYTO107-VA-1) | pCT-Endo-GFP | Endosome | RhoB |
| CMV (CYTO107R-PA-1/CYTO107R-VA-1) | pCT-Endo-RFP | Endosome | RhoB |
| CMV (CYTO108-PA-1/CYTO108-VA-1) MSCV (CYTO108-PB-1/CYTO108-VB-1) | pCT-Pero-GFP | Peroxisome | Peroxisomal consensus signal |
| CMV (CYTO109-PA-1/CYTO109-VA-1) | pCT-Autophago-GFP | Autophagosome | LC3 |
| CMV (CYTO109R-PA-1/CYTO109R-VA-1) | pCT-Autophago-RFP | Autophagosome | LC3 |
| CMV (CYTO120-PA-1/CYTO120-VA-1) | pCT-CD63-GFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO120R-PA-1/CYTO120R-VA-1) | pCT-CD63-RFP | Exosome/Secretory | CD63 Tetraspanin |
| CMV (CYTO122-PA-1/CYTO122-VA-1) | pCT-CD9-GFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO123-PA-1/CYTO123-VA-1) | pCT-CD9-RFP | Exosome/Secretory | CD9 Tetraspanin |
| CMV (CYTO124-PA-1/CYTO124-VA-1) | pCT-CD81-GFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO125-PA-1/CYTO125-VA-1) | pCT-CD81-RFP | Exosome/Secretory | CD81 Tetraspanin |
| CMV (CYTO110-PA-1/CYTO110-VA-1) MSCV (CYTO110-PB-1/CYTO110-VB-1) | pCT-Actin-GFP | Cytoskeleton | β-actin |
| CMV (CYTO111-PA-1/CYTO111-VA-1) MSCV (CYTO111-PB-1/CYTO111-VB-1) | pCT-Tublin-GFP | Cytoskeleton | α-tublin |
| CMV (CYTO111R-PA-1/CYTO111R-VA-1) | pCT-Tublin-RFP | Cytoskeleton | α-tublin |
| CMV (CYTO112-PA-1/CYTO112-VA-1) | pCT-MAP4-GFP | Microtubule | Microtubule associated protein 4 (MAP4) |
| CMV (CYTO113-PA-1/CYTO113-VA-1) MSCV (CYTO113-PB-1/CYTO113-VB-1) | pCT-H2B-GFP | Nucleus | H2B |
| CMV (CYTO114-PA-1/CYTO114-VA-1) | pCT-Apoptosis-Luc | Caspase 3/7 activation | Cyclic inactive luciferase |
| CMV (CYTO115-PA-1/CYTO115-VA-1) | pCT-GFP-BAX | Cytosol to Mitochondria | BAX |
| CMV (CYTO116-PA-1/CYTO116-VA-1) | pCT- Catenin-GFP | Cytosol to Nucleus/Cell Membrane | β-Catenin activation |
| CMV (CYTO117-PA-1/CYTO117-VA-1) | pCT-CMV-PSD95-GFP | Dendrite Membranes | PSD-95 |
| CMV (CYTO118-PA-1/CYTO118-VA-1) MSCV (CYTO118-PB-1/CYTO118-VB-1) | pCT-Cyto-GFP | Cytosolic Compartment | GFP-Untagged |
| CMV (CYTO119-PA-1/CYTO119-VA-1) | pCT-Cyto-RFP | Cytosolic Compartment | RFP-Untagged |
| MSCV (CYTO121-PB-1/CYTO121-VB-1) | pCT-Cyto-GFpLuc | GFP and Luciferase Control | GFP and Luciferase (Untagged) |
How It Works
How It Works
Visualizing the cell with Cyto-Tracers
SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.
Supporting Data
Supporting Data
See some of our Cyto-Tracers in action
FAQs
Citations
-
Kessler, M, et al. (2019) Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nat Commun. 2019; 10(1):1194. PM ID: 30886143
-
Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017; 35(7):1747-1759. PM ID: 28376567
-
Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017; 38(6):592-603. PM ID: 28402394
-
Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. 2017;. Link: Thesis
-
Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017; 292(6):2470-2484. PM ID: 28003368
-
Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016; 37(1):313-21. PM ID: 26211004
-
Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015; 6:7506. PM ID: 26103923
-
Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. 2015;. Link: Thesis
-
Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. 2015;. Link: Thesis
-
VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. 2015;. Link: Thesis
-
Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014; 289(47):32526-37. PM ID: 25261472
-
Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014; 5:5513. PM ID: 25423885
-
Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014; 5:5266. PM ID: 25366663