Exo-Flow96 CD81 IP Kit

For high-throughput, 96-well plate-based CD81 immunopurification of exosomes from serum, plasma, media, or other biofluids
  • Selection of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW96A-CD81 Exo-Flow96 CD81 IP kit 96 Reactions $1611
- +

Overview

Overview

Streamline affinity-based exosome immunopurification

With magnetic beads already pre-coupled to anti-CD81 antibodies and delivered in a 96-well format, SBI’s Exo-Flow96 CD81 IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD81 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.

  • Selection of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation

Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.

Exosome capture is highly efficient with large bead size

Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).

The optional Exo-FlowMag96 simplifies Exo-Flow IP Kit isolations

Different formats for different needs

To facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.

Cat.#Kit
EXOFLOW32A-CD63Exo-Flow32 CD63 IP kit
EXOFLOW32A-CD81Exo-Flow32 CD81 IP kit
EXOFLOW32A-CD9Exo-Flow32 CD9 IP kit
EXOFLOW32A-TetraExo-Flow32 Tetra IP kit (CD9, CD63, CD81)
EXOFLOW96A-CD63Exo-Flow96 CD63 IP kit
EXOFLOW96A-CD81Exo-Flow96 CD81 IP kit
EXOFLOW96A-CD9Exo-Flow96 CD9 IP kit
EXOFLOW96A-TetraExo-Flow96 Tetra IP kit (CD9, CD63, CD81)

How It Works

Supporting Data

Supporting Data

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.

Western Blot showing selective immunopurification using Exo-Flow96 IP Kits

Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.

NanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles and good yieldsNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles

Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.

FAQs

Resources

Citations

  • Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s Disease patients and cognitively normal individuals. medRxiv. 2024;. Link: medRxiv
  • Klemetti, MM, et al. (2024) Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of extracellular vesicles. 2024; 13(2):e12413. PM ID: 38353485
  • Mekala, N, et al. (2024) Alcohol and e-cigarette damage alveolar-epithelial barrier by activation of P2X7r and provoke brain endothelial injury via extracellular vesicles. Cell communication and signaling : CCS. 2024; 22(1):39. PM ID: 38225580
  • Garbin, A, et al. (2023) MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation. Biochemical pharmacology. 2023; 215:115747. PM ID: 37591448
  • Guda, P, et al. (2023) Nanoscopic and functional characterization of keratinocyte-originating exosomes in the wound fluid of non-diabetic and diabetic chronic wound patients. Nano Today. 2023; 52:101954. Link: Nano Today
  • Nakazaki, M, et al. (2023) Human mesenchymal stem-derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical and translational medicine. 2023; 13(6):e1284. PM ID: 37323108
  • Mimmi, S, et al. (2023) SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections. Clinical chemistry and laboratory medicine. 2023;. PM ID: 36972680
  • Bertolini, I, et al. (2023) Intercellular HIF1a reprogams mammary progenitors and myeloid immune evasion to drive high-risk breast lesions. The Journal of clinical investigation. 2023;. PM ID: 36892943
  • Shen, H & Lane, RA. (2023) Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing. bioRxiv : the preprint server for biology. 2023;. PM ID: 36778262
  • Yuan, X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. The American journal of pathology. 2023;. PM ID: 36638912
  • Koken, GY, et al. (2022) Wharton Jelly Derived Mesenchymal Stem Cell’s Exosomes Demonstrate Significant Antileishmanial and Wound Healing Effects in Combination with Aloe-Emodin: An in Vitro Study. Journal of pharmaceutical sciences. 2022;. PM ID: 35995206
  • Luo, Z, et al. (2022) Human bone marrow mesenchymal stem cell-derived extracellular vesicles inhibit shoulder stiffness via let-7a/Tgfbr1 axis. Bioactive Materials. 2022;. Link: Bioactive Materials
  • Nirujogi, TS, et al. (2022) Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of innate immunity. 2022;:1-14. PM ID: 35367992
  • Marra, KV, et al. (2022) Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration. JCI insight. 2022; 7(12). PM ID: 35639473
  • Li, Y, et al. (2022) Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1. Research Square. 2022;. Link: Research Square
  • Mullen, M, et al. (2022) Mechanical strain drives exosome production, function, and miRNA cargo in C2C12 muscle progenitor cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2022;. PM ID: 36250617
  • Beatriz, M, et al. (2022) Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington’s disease. Journal of Extracellular Biology. 2022; 1(10). Link: Journal of Extracellular Biology
  • Zhang, H, et al. (2021) Improving Isolation of Extracellular Vesicles by Utilizing Nanomaterials. Membranes. 2021; 12(1). PM ID: 35054584
  • Luo, ZW, et al. (2021) Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation. World journal of stem cells. 2021; 13(11):1762-1782. PM ID: 34909122
  • Zhang, Z, et al. (2021) Surface Located Adiponectin On Adipocytes-derived Exosomes Mediates Adipocytes/cardiomyocytes Communication And Contributes To Cardioprotection. Circulation. 2021;. Link: Circulation

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW96A-CD81 Exo-Flow96 CD81 IP kit 96 Reactions $1611
- +

Overview

Overview

Streamline affinity-based exosome immunopurification

With magnetic beads already pre-coupled to anti-CD81 antibodies and delivered in a 96-well format, SBI’s Exo-Flow96 CD81 IP Kit simplifies high-throughput, antibody-based exosome isolation. Our magnetic bead-coupled anti-CD81 antibodies are extensively validated, and the high-quality Exo-Flow IP kit components ensure reliable, reproducible affinity-based exosome purification directly from serum or plasma. Exosomes can also be purified from other biofluids such as media, urine, and CSF, but must first be concentrated using either ExoQuick-TC® or ultracentrifugation.

  • Selection of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Available in 32- and 96-well formats for high-throughput, affinity-based exosome isolation

Even better, with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, enabling capture of billions of exosomes from one sample.

Exosome capture is highly efficient with large bead size

Each IP kit comes with all the necessary reagents for immunopurification—capture antibody pre-coupled to magnetic beads in 96-well clear plates (as 8-well strips) with covers, wash buffer, Exosome Elution Buffer, and clearing reagent (to remove Exosome Elution Buffer). A 96-well plate magnet, the Exo-FlowMag96, can be purchased separately (Cat.# EXOFLOWMAG-1).

The optional Exo-FlowMag96 simplifies Exo-Flow IP Kit isolations

Different formats for different needs

To facilitate a range of studies, SBI built the Exo-Flow IP system to be highly modular. We offer a range of magnetic bead-coupled antibodies in 32- and 96-well formats.

Cat.#Kit
EXOFLOW32A-CD63Exo-Flow32 CD63 IP kit
EXOFLOW32A-CD81Exo-Flow32 CD81 IP kit
EXOFLOW32A-CD9Exo-Flow32 CD9 IP kit
EXOFLOW32A-TetraExo-Flow32 Tetra IP kit (CD9, CD63, CD81)
EXOFLOW96A-CD63Exo-Flow96 CD63 IP kit
EXOFLOW96A-CD81Exo-Flow96 CD81 IP kit
EXOFLOW96A-CD9Exo-Flow96 CD9 IP kit
EXOFLOW96A-TetraExo-Flow96 Tetra IP kit (CD9, CD63, CD81)

How It Works

Supporting Data

Supporting Data

See isolation data using Exo-FLOW IP Kits

For these studies, either human serum or HEK293 exosomes concentrated from cell culture media using ExoQuick-TC were added to the antibody-coupled magnetic beads. After washing, exosomes were eluted and recovery estimated using a standard BCA protein assay.

Western Blot showing selective immunopurification using Exo-Flow96 IP Kits

Figure 1. CD63 and CD9, two exosome markers, are readily detected in samples purified using Exo-Flow Kits. Approximately 1 µg of protein was loaded per well on a 4-20% gradient protein PAGE. The proteins were separated and transferred to nitrocellulose membranes for Western blot analysis. The blots were probed with either with anti-CD63 or anti-CD9 antibodies to detect the exosome protein markers.

NanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles and good yieldsNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles

Figure 2. NanoSight analysis shows Exo-Flow IP Kits deliver good yields of particles whose sizes are consistent with exosomes.

FAQs

Citations

  • Boyer, E, et al. (2024) Comparison of plasma soluble and extracellular vesicles-associated biomarkers in Alzheimer’s Disease patients and cognitively normal individuals. medRxiv. 2024;. Link: medRxiv
  • Klemetti, MM, et al. (2024) Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk. Journal of extracellular vesicles. 2024; 13(2):e12413. PM ID: 38353485
  • Mekala, N, et al. (2024) Alcohol and e-cigarette damage alveolar-epithelial barrier by activation of P2X7r and provoke brain endothelial injury via extracellular vesicles. Cell communication and signaling : CCS. 2024; 22(1):39. PM ID: 38225580
  • Garbin, A, et al. (2023) MiR-146a-5p enrichment in small-extracellular vesicles of relapsed pediatric ALCL patients promotes macrophages infiltration and differentiation. Biochemical pharmacology. 2023; 215:115747. PM ID: 37591448
  • Guda, P, et al. (2023) Nanoscopic and functional characterization of keratinocyte-originating exosomes in the wound fluid of non-diabetic and diabetic chronic wound patients. Nano Today. 2023; 52:101954. Link: Nano Today
  • Nakazaki, M, et al. (2023) Human mesenchymal stem-derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical and translational medicine. 2023; 13(6):e1284. PM ID: 37323108
  • Mimmi, S, et al. (2023) SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections. Clinical chemistry and laboratory medicine. 2023;. PM ID: 36972680
  • Bertolini, I, et al. (2023) Intercellular HIF1a reprogams mammary progenitors and myeloid immune evasion to drive high-risk breast lesions. The Journal of clinical investigation. 2023;. PM ID: 36892943
  • Shen, H & Lane, RA. (2023) Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing. bioRxiv : the preprint server for biology. 2023;. PM ID: 36778262
  • Yuan, X, et al. (2023) Contribution of Hepatic Steatosis-Intensified Extracellular Vesicle Release to Aggravated Inflammatory Endothelial Injury in Liver-Specific Asah1 Gene Knockout Mice. The American journal of pathology. 2023;. PM ID: 36638912
  • Koken, GY, et al. (2022) Wharton Jelly Derived Mesenchymal Stem Cell’s Exosomes Demonstrate Significant Antileishmanial and Wound Healing Effects in Combination with Aloe-Emodin: An in Vitro Study. Journal of pharmaceutical sciences. 2022;. PM ID: 35995206
  • Luo, Z, et al. (2022) Human bone marrow mesenchymal stem cell-derived extracellular vesicles inhibit shoulder stiffness via let-7a/Tgfbr1 axis. Bioactive Materials. 2022;. Link: Bioactive Materials
  • Nirujogi, TS, et al. (2022) Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of innate immunity. 2022;:1-14. PM ID: 35367992
  • Marra, KV, et al. (2022) Bioactive extracellular vesicles from a subset of endothelial progenitor cells rescue retinal ischemia and neurodegeneration. JCI insight. 2022; 7(12). PM ID: 35639473
  • Li, Y, et al. (2022) Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1. Research Square. 2022;. Link: Research Square
  • Mullen, M, et al. (2022) Mechanical strain drives exosome production, function, and miRNA cargo in C2C12 muscle progenitor cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2022;. PM ID: 36250617
  • Beatriz, M, et al. (2022) Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington’s disease. Journal of Extracellular Biology. 2022; 1(10). Link: Journal of Extracellular Biology
  • Zhang, H, et al. (2021) Improving Isolation of Extracellular Vesicles by Utilizing Nanomaterials. Membranes. 2021; 12(1). PM ID: 35054584
  • Luo, ZW, et al. (2021) Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation. World journal of stem cells. 2021; 13(11):1762-1782. PM ID: 34909122
  • Zhang, Z, et al. (2021) Surface Located Adiponectin On Adipocytes-derived Exosomes Mediates Adipocytes/cardiomyocytes Communication And Contributes To Cardioprotection. Circulation. 2021;. Link: Circulation