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  • Exosome custom services

Exosome Lipidomics & Metabolomics Service for Deeper Insights from Exosomes

Whether you’re interested in circulating biomarker discovery, basic exosome research, or other exosome-related studies, SBI’s Exosome Lipidomics & Metabolomics Service helps you quickly and efficiently get the most information from your exosomes. Simply send us your sample or purified exosomes and we’ll send back a spreadsheet with putative identifications, mass/charge ratios, and differential analysis (if requested).

What can lipidomics of exosomes tell you?

Lipids are an important part of cellular physiology, and are increasingly being recognized for their importance in exosome biology as well. Exosomes were recently shown to have the highest lipid-to-protein ratio of all classes of extracellular vesicles (1), with lipid content that both differs from the parent cell the vesicles are shed from (2) and also changes as exosomes undergo a variety of physiological processes (3). These unique lipid profiles can serve as novel circulating biomarkers, and recent evidence suggests that specific lipid species carried by exosomes can also modulate the function of recipient cells (4).

With so much information revealed by lipid content, lipidomics studies of exosomes are a great way to identify lipid-based biomarkers and for understanding vesicle biogenesis and function (5).

What can metabolomics of exosomes tell you?

In addition to lipids, exosomes carry a wide range of metabolites that can also be used for biomarker discovery. A recent study found distinctly different populations of metabolites in PANC1 cells treated with TGF-β, a known driver of cancer progression, versus a control group, suggesting that metabolite profiling can be used for differentiating cellular states (6). Like exosomal lipids, exosomal metabolites can also take an active role in the target cell, and have been shown to reprogram metabolic machinery upon uptake by cancer cells, fueling growth (7).

How the service works

Typical timeline: 4–6 weeks

Input sample requirements

Biofluid Volume
Serum 500μl — 1ml
Plasma 500μl — 1ml
Cell Media 5ml — 10ml
Urine 5ml — 10ml
Spinal Fluid 5ml — 10ml
Ascites Fluid 500μl — 1ml
Other Inquire

Our standard service (Catalog no. CSEQ850A-1) offers UPLC-FTMS analysis of both very polar metabolites in the aqueous phase and lipids and other non-polar metabolites in the organic phase. Individual polar or non-polar analysis also available upon request.

Note that all IDs are based only on retention time, accurate mass measurement, and preliminary MS/MS data, and cannot be guaranteed. Not all peaks will be identified, and we recommend that any peaks of interest—either due to the identity of the compound reported and/or change in intensity between samples—MUST be verified by additional targeted analysis at additional cost.

     Download Lipidomics and Metabolomics Service 
Datasheet

Data deliverables

  • MS data will be delivered as Excel files with putative identification based on the m/z ratio of the analytes
  • Differential analysis of lipids and metabolites between treatment groups will be included in the Excel files

Example data

Exosomes were harvested from 300 μL of serum of a healthy human subject and a subject with Acute Myeloid Leukemia (AML). Discovery-based lipidomic/ metabolomics profiling was performed on the serum vesicles from the two subjects.

Pathways represented by metabolites that are enriched in exosomes from the AML subject

Register

Complete this form and you'll instantly have access to the sample data showing enriched and downregulated metabolites in AML patient-derived exosomes, along with all references.

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