Put SBI’s scientists to work for you
High-quality Custom Services
- Custom cloning and virus packaging
- Cas9 and TALEN construction
- Custom iPSCs and stable cell lines
- Large-scale minicircle production
- Exosome custom services
Exosome Characterization by NanoSight Service
Have SBI isolate and characterize exosomes from your biofluid samples (serum, urine, media, CSF, etc.). You can also send SBI exosomes you have already isolated and we can perform the nanoparticle tracking analysis. Each sample will be gently vortexed at 2.5k for 10 sec and then bath sonicated for 10 min at 33°C to ensure adequate exosome dispersion in the solution. NanoSight measurements are carried out in 0.02 um filtered PBS to remove any background and then visualized on an LM10 NanoSight instrument.
Summary reports provided in the service
SBI provides the particle analysis data, measured in triplicate in reports that show the mean, mode diameter size of the exosomes as well as concentration of the exosomes. Each sample is visualized independently and a video recorded of the data collection. This data video is also supplied as a wmv file included in the service. SBI's NanoSight complete service can be ordered using catalog# CSNANO100A-1.
Data collection videos (wmv files)
Shown above are exosomes moving in Brownian motion and the laser light scattering is recorded and each particle tracked.