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cDNA Synthesis Kit

Profile any RNA by real-time qPCR

An all-in-one cDNA synthesis kit

  • Tail and tag all RNAs for cDNA synthesis
  • Obtain full RNA coverage for any purpose
  • Profile any RNA
  • mRNA, miRNA, lncRNA, sn/snoRNA, rRNA

Profile any RNA by real-time qPCR with RNA-Quant

An all-in-one cDNA synthesis kit
For the last few decades of the 20th century, the underlying dogma of molecular biology has been that the purpose of RNA is to direct the assembly of proteins from amino acids. These are the functions of messenger RNAs (mRNAs). mRNAs code for protein genes, have 5'mG caps and most often have poly-A tails. A few exceptions to this paradigm were known (for example, ribosomal RNA and transfer RNA, which are functional RNA macromolecules that do not code for protein, or viral genomes that exist as or pass through an RNA phase as part of total genome replication). There are now numerous exceptions that include: microRNAs, small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs) and long non-coding RNAs (lncRNAs).

Other cDNA synthesis kits only allow for the quantitation of one or two classes of RNAs. The RNA-Quant kit enables the qPCR measurement of many classes of RNA from a single cDNA synthesis. The kit includes 3 endogenous RNA reference qPCR assays as normalization signals: miR-16 microRNA, Y RNA lncRNA and GAPDH mRNA. These three qPCR assays will work with both human and mouse RNA samples. The functions of each class of RNA are briefly summarized in the Table below.

Types of RNA you can profile with RNA-Quant