Mouse pre-miRNA Expression Lentivectors (Lenti-miRs)

Regulate gene expression or study miRNA function with lentivector-delivered precursor miRs that get efficiently and accurately processed into mature miRs

There are 306 individual products in this category.

Show All Products

Overview

Streamline your studies and maximize your options for miR expression

Whether you’re using miRNAs to study cellular processes or develop the next generation of therapeutics, SBI’s comprehensive collection of mouse precursor miRNA lentivectors are a superior alternative to synthetic RNAs. Like synthetic miRs, SBI’s precursor miRNAs can be transfected into target cells for transient miR expression. But unlike synthetic miRs, they can also be transduced into a variety of target cells—including primary cells, stem cells, and other hard-to-transfect cell lines—to create stable miR-producing cell lines, maximizing your options for miR expression.

Why else choose SBI’s precursor miRNA lentivectors?

In addition, SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior. Each construct is cloned with the native stem-loop structure plus an additional 200 – 400 bps of upstream and downstream DNA, ensuring that the miRNA processing machinery operates just as it would with the native transcript.

SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior

And because our collection includes cancer and stem cell-related miRNA clusters, we offer a wider range of constructs than just the mature miRNA sequences listed in miRbase.

The precursor miRNA Expression Lentivectors drive expression of the miR precursor with the constitutive CMV promoter, for strong expression in common cell types including HeLa, HEK293, and HT1080 cell lines. Transductants/transfectants can be easily selected for or sorted using copGFP and puromycin markers.

  • Transfect or transduce the precursor miRNA lentivectors
  • Create stably-expressing miR cell lines
  • Leverage SBI’s highly-regarded lentivectors
  • Obtain accurate miR processing and efficient expression through the use of native precursor sequences
  • Use copGFP and puromycin markers to identify desired clones

How It Works

SBI’s Mouse pre-miRNA Expression Lentivectors use the native miRNA processing machinery

SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behaviorSBI’s Mouse pre-miRNA Expression Lentivectors use the native miRNA processing machinery

Supporting Data

Get high levels of miRs from our Mouse pre-miRNA Expression Lentivectors

Get high levels of miRs from our Mouse pre-miRNA Expression Lentivectors

METHODS: HEK 293 cells were transduced with the mouse miR-302bcad-367 (Cat.# MMIR-302bcad+367-PA-CL) packaged lentivirus. After 48-hours, cellular RNA was analyzed by Real-time qPCR using SBI’s QuantiMir small RNA quantification system (Cat.# RA420A-1). The expression levels of miR-302-b, -c,-a,-d and -367 were measured and compared to non-transduced cellular controls. Robust mature microRNA overexpression was observed in the transduced cells when compared to controls. Cells were also monitored for GFP using fluorescent microscopy.

Available Mouse pre-miRNA Expression Lentivectors (Lenti-miRs)

Use the search box below to find a product by name or catalog number

Search by name or catalog number

Description
Size
Catalog Number
Price
Quantity
Add to Cart
Mouse pre-microRNA Expression Construct mir-100
Bacterial Streak
MMIR-100-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-101a
Bacterial Streak
MMIR-101a-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-101b
Bacterial Streak
MMIR-101b-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-101c
Bacterial Streak
MMIR-101c-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-103-1
Bacterial Streak
MMIR-103-1-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-103-2
Bacterial Streak
MMIR-103-2-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-105
Bacterial Streak
MMIR-105-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-106a
Bacterial Streak
MMIR-106a-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-106b
Bacterial Streak
MMIR-106b-PA-1
$ 558
Mouse pre-microRNA Expression Construct mir-107
Bacterial Streak
MMIR-107-PA-1
$ 558