LncRNA Profiler Complete qPCR Array Kit (Human)

Study the role of lncRNAs in a range of cellular processes – this kit includes reagents for converting lncRNA to cDNA, qPCR primers, and SYBR Green master mix

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LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent)

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LncRNA Profiler Complete qPCR Array Kit (cDNA synthesis kit, qPCR array and SYBR Green reagent)

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20 Profiles
RA910A-1
$ 930
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Overview

Learn more about lncRNAs

Profile 90 human lncRNAs with this kit that includes reagents for converting lncRNAs to cDNAs, a primer set for qPCR assays in a 96-well format, and SYBR Green master mix for your qPCR reactions. Get robust cDNA yields and clean, sensitive qPCR quantitation with undetectable background.

Get robust cDNA yields and clean, sensitive qPCR quantitation with undetectable backgroundLncRNA Profiler qPCR Array Kit (Human)

The kit includes five positive housekeeping reference controls and one negative control. In addition, the kit supports subcellular localization studies through RNA reference controls for the nucleolus, nucleus, and cytoplasm:

  • Nucleolus—snoRNA RNU43 (and some 18S rRNA)
  • Nucleus—small Nuclear splicing snRNA U6B
  • Cytoplasm—GAPDH, Lamin A/C (Human Array) and Beta Actin (Mouse Array) as well as 18S rRNA

The kit supports subcellular localization studies through RNA reference controls for the nucleolus, nucleus, and cytoplasm

How It Works

Using the LncRNA Profiler qPCR Array Kit

First, get your lncRNA ready for qPCR by converting to cDNA

While some lncRNAs have endogenouse polyA tails, other lncRNAs do not. To enhance qPCR assay performance, the cDNA synthesis workflow includes steps and reagents to polyadenylate all lncRNAs before cDNA conversion using the tagged oligo dT adaptor and random primers. This combined RNA tailing and oligo dT plus random primers boosts cDNA yield significantly and enables strand-specific lncRNA qPCR profiling.

  • Tag all lncRNAs with a poly-A tail
  • Anneal an oligo-dT adaptor
  • Reverse transcribe to create first-strand cDNA

The result is a cDNA pool of anchor-tailed lncRNAs that are ready for qPCR.

Then conduct qPCR assays

Get additional information about the array including lncRNA sequences, LncRNA database links, and qPCR analysis templates in the Excel document, “Assay List and Free Analysis Software for Human LncProfiler qPCR Array

Supporting Data

Example LncRNA Profiler qPCR Array Kit (Human) Data

Sample dissociation analysis curves from the human lncRNA Profiler qPCR Array Kit

Figure 1. Sample dissociation analysis curves.

Compare lncRNA expression in normal human fibroblasts and fibrosarcoma cells

Figure 2. Compare lncRNA expression in normal human fibroblasts and fibrosarcoma cells. LncRNAs known to be upregulated in cancer, such as Neat1, Anril, Hotair and Malat1, were all detected at highly elevated levels in the fibrosarcoma cells (HT1080) when compared to the normal fibroblast cells (HFF). The data are plotted as normalized expression levels.


Product Documentation

Citations

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  • Zhang, J, et al. (2017) Curcumin suppresses cisplatin resistance development partly via modulating extracellular vesicle-mediated transfer of MEG3 and miR-214 in ovarian cancer. Cancer Chemother. Pharmacol.. 2017 Mar 1; 79(3):479-487. PM ID: 28175963
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  • Qi, P, Zhou, XY & Du, X. (2016) Circulating long non-coding RNAs in cancer: current status and future perspectives. Mol. Cancer. 2016 May 17; 15(1):39. PM ID: 27189224
  • Villamizar, O, et al. (2016) Data in Brief. Data in Brief. 2016 Apr 7; 7:1288–1295. Link: Data in Brief
  • Villamizar, O, et al. (2016) Fas-antisense long noncoding RNA is differentially expressed during maturation of human erythrocytes and confers resistance to Fas-mediated cell death. Blood Cells, Molecules, and Diseases. 2016 Mar 3; 58:57-66. Link: Blood Cells, Molecules, and Diseases
  • Kang, Y, et al. (2016) PCGEM1 stimulates proliferation of osteoarthritic synoviocytes by acting as a sponge for miR-770. J. Orthop. Res.. 2016 Mar 1; 34(3):412-8. PM ID: 26340084
  • Thorenoor, N, et al. (2016) Long non-coding RNA ZFAS1 interacts with CDK1 and is involved in p53-dependent cell cycle control and apoptosis in colorectal cancer. Oncotarget. 2016 Jan 5; 7(1):622-37. PM ID: 26506418
  • Carter, G, et al. (2015) Circulating long noncoding RNA GAS5 levels are correlated to prevalence of type 2 diabetes mellitus. BBA Clin. 2015 Dec 1; 4:102-7. PM ID: 26675493
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  • Shen, J, et al. (2015) Exploration of Deregulated Long Non-Coding RNAs in Association with Hepatocarcinogenesis and Survival. Cancers (Basel). 2015 Sep 10; 7(3):1847-62. PM ID: 26378581
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