Human pre-miRNA Scramble Negative Control Expression Lentivector

Easily get the confidence of a good negative control for your human pre-miRNA Lentivector studies with this scrambled, non-targeting pre-miRNA Lentivector

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Scramble control hairpin in pCDH-CMV-MCS-EF1α-copGFP (CD511B-1)

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Scramble control hairpin in pCDH-CMV-MCS-EF1α-copGFP (CD511B-1)

[variation_id] => 64654 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
Bacterial Streak
PMIRH000PA-1
$ 533

Scramble control hairpin in pCDH-CMV-MCS-EF1α-copGFP (CD511B-1) pre-packaged virus

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Scramble control hairpin in pCDH-CMV-MCS-EF1α-copGFP (CD511B-1) pre-packaged virus

[variation_id] => 64655 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
1x10^6 IFUs
PMIRH000VA-1
$ 644
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Overview

Supporting your studies with ready-to-go negative controls

No need to make a negative control for your Human Precursor miRNA Expression Lentivector Studies—SBI’s already built one for you. With the Human pre-miRNA Scramble Negative Control Expression Lentivector, you get the same lentivector that expresses our targeted miRNAs, but with a scrambled, non-targeting stem-loop sequence so you can easily control for increased miRNA effects.

The Human pre-miRNA Scramble Negative Control Expression Lentivector is available as both ready-to-transfect lentivector plasmid and ready-to-transduce pre-packaged lentivirus.

Why choose SBI’s precursor miRNA lentivectors?

Whether you’re using miRNAs to study cellular processes or develop the next generation of therapeutics, SBI’s comprehensive collection of Lenti-miRsTM—human precursor miRNA lentivectors—are a superior alternative to synthetic RNAs. Like synthetic miRs, SBI’s Lenti-miRs can be transfected into target cells for transient miR expression. But unlike synthetic miRs, they can also be transduced into a variety of target cells—including primary cells, stem cells, and other hard-to-transfect cell lines—to create stable miR-producing cell lines, maximizing your options for miR expression.

In addition, SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior. Each construct is cloned with the native stem-loop structure plus an additional 200 – 400 bps of upstream and downstream DNA, ensuring that the miRNA processing machinery operates just as it would with the native transcript.

SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior

And because our collection includes cancer and stem cell-related miRNA clusters, we offer a wider range of constructs than just the mature miRNA sequences listed in miRbase.

The precursor miRNA Expression Lentivectors drive expression of the miR precursor with the constitutive CMV promoter, for strong expression in common cell types including HeLa, HEK293, and HT1080 cell lines. Transductants/transfectants can be easily sorted using copGFP.

  • Transfect or transduce the precursor miRNA lentivectors
  • Create stably-expressing miR cell lines
  • Leverage SBI’s highly-regarded lentivectors
  • Obtain accurate miR processing and efficient expression through the use of native precursor sequences
  • Use copGFP markers to identify desired clones

How It Works

SBI’s Human pre-miRNA Expression Lentivectors use the native miRNA processing machinery

SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behaviorSBI’s Human pre-miRNA Expression Lentivectors use the native miRNA processing machinery

Supporting Data

Get high levels of miRs from our Human pre-miRNA Expression Lentivectors

Lenti-miR-205 is expressed at high levels in MCF-7 cells

Lenti-miR-205 is expressed at high levels in MCF-7 cells

Lenti-miRs are overexpressed in HEK293 cellsLenti-miRs are overexpressed in HEK293 cells

Lenti-miR stem cell cluster constructs are overexpressed Lenti-miR stem cell cluster constructs are overexpressed

Lenti-miR stem cell cluster constructs are overexpressed Lenti-miR stem cell cluster constructs are overexpressed


Citations

  • Van Roosbroeck, K, et al. (2017) Combining Anti-Mir-155 with Chemotherapy for the Treatment of Lung Cancers. Clin. Cancer Res.. 2017 Jun 1; 23(11):2891-2904. PM ID: 27903673
  • Zangari, J, et al. (2017) Rapid decay of engulfed extracellular miRNA by XRN1 exonuclease promotes transient epithelial-mesenchymal transition. Nucleic Acids Res.. 2017 Apr 20; 45(7):4131-4141. PM ID: 27994032
  • Li, Y, et al. (2017) miR-124 represses the mesenchymal features and suppresses metastasis in Ewing sarcoma. Oncotarget. 2017 Feb 7; 8(6):10274-10286. PM ID: 28055964
  • Abruzzese, MP, et al. (2016) Inhibition of bromodomain and extra-terminal (BET) proteins increases NKG2D ligand MICA expression and sensitivity to NK cell-mediated cytotoxicity in multiple myeloma cells: role of cMYC-IRF4-miR-125b interplay. J Hematol Oncol. 2016 Dec 1; 9(1):134. PM ID: 27903272
  • Ishihara, Y, et al. (2016) Tumor-suppressive effects of atelocollagen-conjugated hsa-miR-520d-5p on un-differentiated cancer cells in a mouse xenograft model. BMC Cancer. 2016 Jul 7; 16:415. PM ID: 27388711
  • Chen, PY, et al. (2016) Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016 Jul 1; 8(7):712-28. PM ID: 27189169
  • Hou, C, et al. (2016) MicroRNA-31 inhibits lung adenocarcinoma stem-like cells via down-regulation of MET-PI3K-Akt signaling pathway. Anticancer Agents Med Chem. 2016 Mar 10; 16(4):501-18. PM ID: 26299665
  • Colangelo, T, et al. (2016) The miR-27a-calreticulin axis affects drug-induced immunogenic cell death in human colorectal cancer cells. Cell Death Dis. 2016 Feb 25; 7:e2108. PM ID: 26913599
  • Colangelo, T, et al. (2016) Proteomic screening identifies calreticulin as a miR-27a direct target repressing MHC class I cell surface exposure in colorectal cancer. Cell Death Dis. 2016 Feb 25; 7:e2120. PM ID: 26913609
  • 林, 其, et al. (2015) MicroRNA-17-5p Regulation of Apoptosis-Related Protein Expressions in Oral Squam ous Cell Ca rcinoma Cells Is Related to Betel Quid Chewing. 放射治療與腫瘤學. 2015 Dec 1; 22(4):255 - 266. Link: 放射治療與腫瘤學
  • Srinivas, C, et al. (2015) Novel Etoposide Analogue Modulates Expression of Angiogenesis Associated microRNAs and Regulates Cell Proliferation by Targeting STAT3 in Breast Cancer. PLoS ONE. 2015 Nov 10; 10(11):e0142006. PM ID: 26551008
  • Li, J, Lam, M & , . (2015) Registered report: the microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44. Elife. 2015 Jul 31; 4:e06434. PM ID: 26231042
  • Gao, J, et al. (2015) miR-34a-5p suppresses colorectal cancer metastasis and predicts recurrence in patients with stage II/III colorectal cancer. Oncogene. 2015 Jul 30; 34(31):4142-52. PM ID: 25362853
  • Al Akoum, C, et al. (2015) NFAT-1, Sp-1, Sp-3, and miR-21: New regulators of chemokine C receptor 7 expression in mature human dendritic cells. Hum. Immunol.. 2015 May 1; 76(5):307-17. PM ID: 25797200
  • Falkenberg, N, et al. (2015) Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221. Oncotarget. 2015 Apr 10; 6(10):8103-14. PM ID: 25797271
  • Hanniford, D, et al. (2015) Identification of metastasis-suppressive microRNAs in primary melanoma. J. Natl. Cancer Inst.. 2015 Mar 1; 107(3). PM ID: 25677173
  • Yu, SH, et al. (2015) miR-99a suppresses the metastasis of human non-small cell lung cancer cells by targeting AKT1 signaling pathway. J. Cell. Biochem.. 2015 Feb 1; 116(2):268-76. PM ID: 25187230
  • Wong, CC, et al. (2014) Switching of pyruvate kinase isoform L to M2 promotes metabolic reprogramming in hepatocarcinogenesis. PLoS ONE. 2014 Dec 27; 9(12):e115036. PM ID: 25541689
  • Zhang, R, et al. (2014) MicroRNA-377 inhibited proliferation and invasion of human glioblastoma cells by directly targeting specificity protein 1. Neuro-oncology. 2014 Nov 1; 16(11):1510-22. PM ID: 24951112
  • Jebbawi, F, et al. (2014) A microRNA profile of human CD8(+) regulatory T cells and characterization of the effects of microRNAs on Treg cell-associated genes. J Transl Med. 2014 Aug 6; 12:218. PM ID: 25090912