miRZip™ anti-miRNA Expression Lentivectors

Study miRNA function by permanently knocking-down activity of a specific miRNA

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Overview

Gain a thorough understanding of miR function

Permanently knockdown miRs with SBI’s miRZip™ anti-miRNA Expression Lentivectors. Leveraging SBI’s powerful and well-regarded third-generation lentivector technology, each of our miRZip lentivectors expresses a short hairpin RNA (shRNA) that, after processing, preferentially produces an anti-sense miRNA. The hairpin is rationally designed to be asymmetric, ensuring that the sense strand does not contain the endogenous miRNA sequence and enabling accumulation of the anti-miRNA. The result is robust derepression of the transcripts targeted by the microRNA being “zipped,” and elevation of the corresponding protein levels.

SBI’s miRZip Lentivectors are designed for efficient production of anti-miRNAs

Study miRs with SBI’s powerful miRZip technology:

  • Stable and permanent anti-microRNA expression from a constitutive H1 promoter
  • Rationally designed, asymmetric hairpins optimized for anti-sense microRNA production
  • Efficient suppression of specific endogenous microRNAs
  • Reliable delivery to dividing or non-dividing cells
  • Selection/sorting for transfected/transduced cells with either copGFP or puromycin

Conduct high-throughput phenotypic screens with our pool of pre-packaged lentiviral particles containing our entire miRZip collection (Cat.# MZIPPLVA-1).

Supporting Data

See SBI’s mirZip technology in action

miRZip constructs express high levels of anti-microRNAs

miRZip constructs express high levels of anti-microRNAs

Figure 1. miRZip constructs express high levels of anti-microRNAs. miRZip constructs were transfected into HEK293 cells, total RNA isolated, and miRZip levels measured using SBI’s QuantiMir Assay (Cat.# RA420A-1). Compared to QuantiMir assays performed on RNA isolated from non-transfected cells, the miRZip-transfected cells delivered high expression levels of the specific miRZip anti-miRNAs.

Efficient modulation of target protein levels by SBI’s Lenti-miR and miRZip technologies Target protein levels are efficiently modulated by SBI

Figure 2. Target protein levels are efficiently modulated by SBI’s Lenti-miR-29a and miRZip-29a microRNA constructs.

miRZip technology is effective in a functional assaymiRZip-21 and miRZip-145 who anti-oncogenic and oncogenic activity, respectively, in a cell invasion assay using MDA-MB-231 breast cancer cellsmiRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc

Figure 3. miRZip-21 and miRZip-145 exhibit anti-oncogenic and oncogenic activity, respectively, in a cell invasion assay using MDA-MB-231 breast cancer cells. (Top panel) miR-21 is an oncogenic miRNA and miR-145 is a tumor suppressing miRNA. Transduction of miRZip-21 (the anti-miR21 lentiviral construct) and miRZip-145 (the anti-miR145 lentiviral construct), inhibited the respective miRs, resulting in a lower percentage of invading cells in miRZip-21-transduced cells and a higher percentage of invading cells in miRZip-145-transduced cells. (Bottom panel) Additionally, the miRZip-145 lentivirus inhibited endogenous miR-145 and elevated protein expression levels of the miR-145 target oncogene c-Myc.

Available miRZip™ anti-miRNA Expression Lentivectors

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Description
Size
Catalog Number
Price
Quantity
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product-1100 mL
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$ 1

product-1250 mL
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$ 222222

product-150 Assays
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$ 3434343

product-2100 mL
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$ 121

product-2250 mL
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$ 333

product-250 Assays
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$ 677777
ExoMS™ Surface Protein Capture Kit (Tissue Culture)
8 Reactions
EXOMS111A-8
$ 944
test thing
100 mL
9999999999-1
$ 0
AAVanced Concentration Reagent
100 mL
AAV100A-1
$ 423
AAVanced Concentration Reagent
250 mL
AAV110A-1
$ 852