Human pre-miRNA Expression Lentivectors (Lenti-miRs)

Regulate gene expression or study miRNA function with lentivector-delivered precursor miRs that get efficiently and accurately processed into mature miRs

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Overview

Streamline your studies and maximize your options for miR expression

Whether you’re using miRNAs to study cellular processes or develop the next generation of therapeutics, SBI’s comprehensive collection of Lenti-miRs™—human precursor miRNA lentivectors—are a superior alternative to synthetic RNAs. Like synthetic miRs, SBI’s Lenti-miRs can be transfected into target cells for transient miR expression. But unlike synthetic miRs, they can also be transduced into a variety of target cells—including primary cells, stem cells, and other hard-to-transfect cell lines—to create stable miR-producing cell lines, maximizing your options for miR expression.

Why else choose SBI’s precursor miRNA lentivectors?

In addition, SBI’s Lenti-miRs are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior. Each construct is cloned with the native stem-loop structure plus an additional 200 – 400 bps of upstream and downstream DNA, ensuring that the miRNA processing machinery operates just as it would with the native transcript.

SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behavior

And because our collection includes cancer and stem cell-related miRNA clusters, we offer a wider range of constructs than just the mature miRNA sequences listed in miRbase.

The precursor miRNA Expression Lentivectors drive expression of the miR precursor with the constitutive CMV promoter, for strong expression in common cell types including HeLa, HEK293, and HT1080 cell lines. Transductants/transfectants can be easily sorted using copGFP.

  • Transfect or transduce the precursor miRNA lentivectors
  • Create stably-expressing miR cell lines
  • Leverage SBI’s highly-regarded lentivectors
  • Obtain accurate miR processing and efficient expression through the use of native precursor sequences
  • Use copGFP and puromycin markers to identify desired clones

How It Works

SBI’s Human pre-miRNA Expression Lentivectors use the native miRNA processing machinery

SBI’s precursor miRNA lentivectors are designed for efficient expression and accurate processing into mature miRNAs, for native miR-like behaviorSBI’s Human pre-miRNA Expression Lentivectors use the native miRNA processing machinery

Supporting Data

Get high levels of miRs from our Human pre-miRNA Expression Lentivectors

Lenti-miR-205 is expressed at high levels in MCF-7 cells

Lenti-miR-205 is expressed at high levels in MCF-7 cells

Lenti-miRs are overexpressed in HEK293 cellsLenti-miRs are overexpressed in HEK293 cells

Lenti-miR stem cell cluster constructs are overexpressed Lenti-miR stem cell cluster constructs are overexpressed

Lenti-miR stem cell cluster constructs are overexpressed Lenti-miR stem cell cluster constructs are overexpressed

Available Human pre-miRNA Expression Lentivectors (Lenti-miRs)

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Description
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product-1100 mL
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$ 1

product-1250 mL
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$ 222222

product-150 Assays
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$ 3434343

product-2100 mL
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$ 121

product-2250 mL
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$ 333

product-250 Assays
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$ 677777
ExoMS™ Surface Protein Capture Kit (Tissue Culture)
8 Reactions
EXOMS111A-8
$ 944
test thing
100 mL
9999999999-1
$ 0
AAVanced Concentration Reagent
100 mL
AAV100A-1
$ 423
AAVanced Concentration Reagent
250 mL
AAV110A-1
$ 852