Robust, controlled transgene expression with a non-integrating lentiviral system
Combining all the advantages of lentiviral vectors with the benefits of episomal expression, SBI’s pPACK-ID Integrase-defective Lentiviral Packaging Mix is a great option for researchers who want to express transgenes in mammalian cells.
Based on SBI’s popular pPACKH1 lentiviral packaging system, pPACK-ID contains targeted mutations in the POL gene which codes for the integrase protein1. The result is a powerful lentiviral packaging system that is completely compatible with the full range of SBI’s lentiviral vectors, but without integration of the vector into the host genome. This enables transient gene expression in dividing cells and stable expression in quiescent or non-dividing cells. In addition, for researchers interested in gene therapy studies, this system supports transgene expression via lentiviral vectors without the potential risks of insertional mutagenesis.
- Safer—avoids the risks of insertional mutagenesis
- Controlled—transient expression in dividing cells, stable expression in quiescent cells
- Flexible—infect a wide range of cells with the VSV-G pseudotyped envelope
- Optimized—well-characterized virus production based on SBI’s proven pPACK system
- Ready-to-use—fully compatible with all third-generation lentiviral vectors and downstream virus concentration methods (PEG-it, ultracentrifugation, and ultrafiltration)
The pPACK-ID Integrase-defective Lentiviral Packaging Mix consists of an optimized mixture of three plasmids: pPACK-ID-GAG, pPACK-ID-REV, and pVSV-G1.
- The pPACK-ID-GAG plasmid contains the structural (Gag), and replication (Pol) genes which code for some of the proteins required to produce the lentivirus. It also encodes the viral Env gene, which encodes the envelope protein that defines the tropism (e. the range of infectable cells). The defective integrase is also located on this plasmid.
- The pPACK-ID-REV plasmid contains the regulatory protein Rev that is required for HIV replication.
- The pVSV-G plasmid expresses the envelope glycoprotein of vesicular stomatitis virus (VSV-G) from the CMV promoter. VSV-G pseudotyped viral particles mediate viral entry through lipid binding and plasma membrane fusion and can infect both mammalian and non-mammalian cells.
- Sengupta, et al. An Optimized Protocol for Packaging Pseudotyped Integrase Defective Lentivirus. Biol Proced Online. July 11, 2016. 18:14. PMCID: PMC4939624.