Global UltraRapidTM Lentiviral Titering Kit

Rapidly determine pseudovirus titers by measuring the copy number of integrated lentiviral constructs—a faster, simpler workflow than ELISA-based kits

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UltraRapid Lentiviral Global Titering Kit (Human and Mouse compatible)

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UltraRapid Lentiviral Global Titering Kit (Human and Mouse compatible)

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42 Reactions
LV961A-1
$ 528
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Overview

When you need to know—quickly and easily determine lentiviral titers

With a fast and simple workflow, the Global UltraRapidTM Lentiviral Titering Kit, delivers critical lentiviral titer information so you can move more quickly to the next step, whether that’s transduction of target cells in your own lab or delivery of packaged virus particles to another lab.

  • Fast—get data more quickly than what’s possible with conventional p24 ELISA titer kits (< 3 hours from cells-to-titer!)
  • Accurate—relies on highly quantitative qPCR measurement of lentiviral titers
  • Easy—isolation and/or concentration of genomic DNA is NOT required
  • Quantitative—built-in reference control simplifies normalization.
  • Dynamic—obtain titering quantitation up to 15 MOI
  • Compatible—works with most WPRE-containing lenti-constructs (g. pLVCT-H1, pLVTHM, pGIPz, HiPerform™ and all SBI lentivectors)

How It Works

Directly measure infectivity with no background from inactive virus

The Global UltraRapid Lentiviral Titer Kit rapidly determines the titer of pseudovirus particles by measuring the copy number of integrated lentiviral constructs in transduced target cells. By comparing the ratio of endogenous Ultra Conserved Region 1 (UCR1) DNA elements:lentivector-specific WPRE elements to a calibration curve generated with supplied standards, the Kit delivers highly quantitative measurement of the infectivity of the pseudoviral preparation.

Compatible with most WPRE-containing lentivector constructs—including pLVCT-H1, pLVTHM, pGIPz, HiPerform™ and all SBI lentivectors—the Kit contains all of the components needed to measure the number of endogenous UCR1 DNA elements and pseudo-lentiviral-specific WPRE elements. Even better, because the UCR1 primers are completely compatible with both human and mouse genomes, you can titer from either cell type.

The workflow is quick and simple:

  1. Lyse infected cells (2 minutes at 95°C)
  2. Add qPCR Mastermix from the Kit to the cleared lysate
  3. Run a real-time PCR reaction (2 hours)
  4. Analyze results

Supporting Data

Sample data from different Global UltraRapid Viral Titering Kit experiments

Amplification plot of UCR1 genomic DNA from the included calibration standards

Figure 1. Amplification plot of UCR1 genomic DNA from the included calibration standards.

Dissociation curves of the amplified UCR1 products

Figure 2. Dissociation curves of the amplified UCR1 products.

Amplification plot of the WPRE DNA from the calibration standards

Figure 3. Amplification plot of the WPRE DNA from the calibration standards.

Dissociation curves of the amplified WPRE products

Figure 4. Dissociation curves of the amplified WPRE products.

Amplification plot of a sample experiment

Figure 5. Amplification plot of a sample experiment.


Citations

  • Gong, H, et al. (2017) Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells. Sci Rep. 2017 Feb 15; 7:42127. PM ID: 28198371
  • Wang, H, et al. (2016) Expression of recombinant human lysozyme in transgenic chicken promotes the growth of Bifidobacterium in the intestine and improves postnatal growth of chicken. AMB Express. 2016 Dec 1; 6(1):110. PM ID: 27830497
  • Hjelm, BE, et al. (2016) Mifepristone-inducible transgene expression in neural progenitor cells in vitro and in vivo. Gene Ther.. 2016 May 1; 23(5):424-37. PM ID: 26863047
  • Kondoh, K, et al. (2016) A specific area of olfactory cortex involved in stress hormone responses to predator odours. Nature. 2016 Apr 7; 532(7597):103-6. PM ID: 27001694
  • Liu, T, et al. (2015) Oviduct-specific expression of human neutrophil defensin 4 in lentivirally generated transgenic chickens. PLoS ONE. 2015 May 29; 10(5):e0127922. PM ID: 26020529
  • Ivacik, D, et al. (2015) Sustained inhibition of hepatitis B virus replication in vivo using RNAi-activating lentiviruses. Gene Ther.. 2015 Feb 1; 22(2):163-71. PM ID: 25338920
  • Yalvac, ME, et al. (2014) VIP-expressing Dendritic Cells Protect Against Spontaneous Autoimmune Peripheral Polyneuropathy.. Mol. Ther.. 2014 Jul 1; 22(7):1353-1363. PM ID: 28153175
  • Hsu, YC, et al. (2014) Signaling adaptor protein SH2B1 enhances neurite outgrowth and accelerates the maturation of human induced neurons. Stem Cells Transl Med. 2014 Jun 1; 3(6):713-22. PM ID: 24736401
  • Sundarasetty, BS, et al. (2013) Lentivirus-induced dendritic cells for immunization against high-risk WT1(+) acute myeloid leukemia. Hum. Gene Ther.. 2013 Feb 1; 24(2):220-37. PM ID: 23311414
  • Lesch, HP, et al. (2011) Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Ther.. 2011 Jun 1; 18(6):531-8. PM ID: 21248790
  • Jirmo, AC, et al. (2010) Monocytes transduced with lentiviral vectors expressing hepatitis C virus non-structural proteins and differentiated into dendritic cells stimulate multi-antigenic CD8+ T cell responses. Vaccine. 2009 Nov 18; 28(4):922-933. Link: Vaccine
  • Lesch, HP, et al. (2009) Avidin fusion protein-expressing lentiviral vector for targeted drug delivery. Hum. Gene Ther.. 2009 Aug 1; 20(8):871-82. PM ID: 19419273
  • Ivacik - 2014, D. (2014) Developing use for recombinant lentiviral vectors for delivery of anti-hepatitis B virus micro RNA mimics. Thesis. ;. Link: Thesis