Get the critical information you need to move forward with confidence
With SBI’s pPS-EF1α-GFP-RFP Positive Transduction Control Lentivector & Pre-packaged Virus, you can easily optimize transduction conditions for your specific cells by using GFP and/or RFP to image and measure transduction efficiencies. Available as ready-to-transduce, pre-packaged virus or ready-to-transfect lentivector, the pPS-EF1α-GFP-RFP Lentivector uses the moderate EF1α promoter to co-express copepod GFP and RFP. A T2A element mediates co-expression.
Use the right promoter for your positive transduction control
If the EF1α promoter does not provide the best expression levels for your positive control, you can choose from one of SBI’s other pPS-XXX-GFP-RFP Positive Transduction Control Lentivectors & Pre-packaged Viruses.
Promoter
Expression Level
Application
CMV
High
Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV
High
Hematopoietic and stem cells
EF1α
Medium
Most cell types including primary cells and stem cells
PGK
Medium
Most cell types including primary cells and stem cells
UbC
Low
Most cell types including primary cells and stem cells
Supporting Data
SBI’s range of Positive Transduction Control Lentivectors & Pre-packaged Viruses
Figure 1. SBI offers a range of positive transduction controls that enable quantification of transduction efficiency. GFP and RFP are co-expressed from the promoter of your choice.
