Mouse RORγt Promoter GreenFire Lentivector with Puro

Study the balance between tolerance and inflammation with this vector that uses the mRORγt promoter to drive both GFP and luciferase expression (with puro)

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Mouse RORyt Promoter GreenFire lentivector plasmid (with Puro marker)

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Mouse RORyt Promoter GreenFire lentivector plasmid (with Puro marker)

[variation_id] => 65673 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
TCL810A-1
$ 591
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Overview

Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse RORγt Promoter GreenFire Lentivector you can combine SBI’s well-regarded and high quality lentivector technology with a RORγt promoter-reporter architecture that enables easy and quantitative monitoring of RORγt expression. The RORγt promoter drives coordinated expression of GFP and luciferase reporters. The vector also includes a puromycin marker for selection.

Mouse RORγt Promoter GreenFire Lentivector with puro

The promoter sequences and design are based upon Dang, et al.1, Lazarevic, et al.2, and Ruan, et al3.

References

  1. Dang, EV, et al. Nuclear factor-κB in immunity and inflammation: the Treg and Th17 connection. Adv Exp Med Biol. 2012; 946:207-21. PMID: 21948370.
  2. Lazarevic, V. et al. T-bet represses T(H)17 differentiation by preventing Runx1-mediated activation of the gene encoding RORγt. Nat Immunol. 2011 Jan; 12(1):96-104. PMCID: PMC3077962.
  3. Ruan, Q, et al. The Th17 immune response is controlled by the Rel-RORγ-RORγ T transcriptional axis. J Exp Med. 2011 Oct 24; 208(11):2321-33. PMCID: PMC3201209.

Supporting Data

Luciferase activation in response to RORγt transcriptional activators

SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators

Figure 1. SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators. METHODS: Human Jurkat T cells were transduced with lentivirus for the Foxp3, RORγt and IL-17 promoter reporters. Transcription activation was tested using stimulation through the addition of with PMA (5 ng/ml) and Ionomycin (500 ng/ml). Cells were harvested after 24 hours and luciferase activity measured for transcription activation responses.