Mouse IL-17 Promoter GreenFire Lentivector with Puro

Study the balance between tolerance and inflammation with this vector that uses the mIL-17 promoter to drive both GFP and luciferase expression (with Puro)

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Mouse IL-17 Promoter GreenFire lentivector plasmid (with Puro marker)

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Mouse IL-17 Promoter GreenFire lentivector plasmid (with Puro marker)

[variation_id] => 65669 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
TCL710A-1
$ 591
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Overview

Monitor the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the Mouse IL-17 Promoter GreenFire Lentivector with Puro you can combine SBI’s well-regarded and high quality lentivector technology with a IL-17 promoter-reporter architecture that enables easy and quantitative monitoring of IL-17 expression. The IL-17 promoter drives coordinated expression of GFP and luciferase reporters. The vector also includes a puromycin marker for selection.

Mouse IL-17 Promoter GreenFire Lentivector with Puro

The promoter sequences and design are based upon Zhang, et al. (2008)1.

References

  1. Zhang F, et al. Interactions among the transcription factors Runx1, RORγt and Foxp3 regulate the differentiation of interleukin 17-producing T cells. Nat Immunol. 2008 Nov; 9(11):1297-306. PMCID: PMC4778724.

Supporting Data

Luciferase activation in response to IL-17 transcriptional activators

SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators

Figure 1. SBI’s GreenFire Lentivector reporters for mFoxp3, mRORγt, and mIL-17 show strong luciferase activity in response to known transcriptional activators. METHODS: Human Jurkat T cells were transduced with lentivirus for the Foxp3, RORγt and IL-17 promoter reporters. Transcription activation was tested using stimulation through the addition of with PMA (5 ng/ml) and Ionomycin (500 ng/ml). Cells were harvested after 24 hours and luciferase activity measured for transcription activation responses.