Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer
Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With the CuO-Mouse Foxp3-IRES-GFP-EF1α-CymR-T2A-Puro Cumate-Inducible Foxp3 Lentivector, you can easily study the effects of different Foxp3 expression levels with SBI’s titratable, cumate-responsive SparQTM Cumate Switch System. This vector co-expresses the Treg Foxp3 and a GFP marker from the inducible cumate switch promoter, enabling measurement of induction by GFP fluorescence. Co-expression is mediated by an IRES element. The vector also constitutively co-expresses CymR and a puromycin resistance marker from an EF1α promoter, with co-expression mediated by the T2A element.
What’s makes SBI’s SparQ Cumate Switch System so powerful?
With SBI’s SparQTM Cumate Switch System, you can get inducible gene expression in mammalian cells through the binding of cumate, a non-toxic small molecule, to CymR. Expression levels of your gene-of-interest are tightly controlled and increase with increasing cumate concentration until maximum induction is reached—see as much as a 32-fold increase in gene expression. Even better, induction is reversible, so you can turn expression levels on and off. Delivering negligible background expression in the absence of cumate, the SparQ System delivers controlled levels of gene expression.
- Robust—increase expression up to 32-fold
- Adjustable—tune expression levels by titrating the amount of cumate
- Reversible—turn expression on, then off, then on again
- Powerful—suitable for in vivo applications