Mouse α-Tubulin pGreenZeo Differentiation Reporter

Study neurogenesis by expressing GFP in cells where the α-tubulin promoter/enhancer is active

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Mouse Alpha-Tubulin Differentiation Reporter (pGreenZeo, Plasmid)

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Mouse Alpha-Tubulin Differentiation Reporter (pGreenZeo, Plasmid)

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10 µg
SR10025PA-1
$ 1495

Mouse Alpha-Tubulin Differentiation Reporter (pGreenZeo, Virus)

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Mouse Alpha-Tubulin Differentiation Reporter (pGreenZeo, Virus)

[variation_id] => 65489 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x 10^6 IFUs
SR10025VA-1
$ 621
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Overview

Monitor neurogenesis in real time

With SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Mouse α-Tubulin pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin resistance from the mouse α-tubulin promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin.

  • Create stable neurogenesis-reporting cell lines
  • Monitor multiple lineages simultaneously
  • Track differentiation in live cells in real time

Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Choose the differentiation reporter that’s right for you

Neural differentiation reporters

Catalog # Target cell type Species Promoter/enhancer element Official name Additional names
SR1008PA/VA-1 Macrophage, microglia Mouse Cd68 Cd68 macrosialin, Scard1, gp110
SR10015PA/VA-1 Astrocyte Human GFAP GFAP Glial fibrillary acidic protein
SR10016PA/VA-1 Astrocyte Mouse Gfap Gfap Glial fibrillary acidic protein
SR10017PA/VA-1 Microglia Human CD11b ITGAM integrin, alpha M, CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A
SR10018PA/VA-1 Microglia Mouse EMR1 Emr1 EGF-like module containing, mucin-like, hormone receptor-like sequence 1, EGF-TM7, F4/80
SR10019PA/VA-1 Microglia Mouse Iba-1 Aif1 allograft inflammatory factor 1 (Aif-1), ionized calcium binding adapter molecule 1 (Iba-1)
SR10020PA/VA-1 Muller glia Mouse Cd44 Cd44 HERMES, Ly-24, Pgp-1
SR10021PA/VA-1 Neuron Human BM88 Cend1 cell cycle exit and neuronal differentiation 1 (Cend1)
SR10022PA/VA-1 Neuron Mouse Camk2a Camk2a CaMK II; alpha-CaMKII
SR10023PA/VA-1 Neuron Mouse GAD67 Gad1 glutamic acid decarboxylase 1, EP10, GAD67, Gad-1
SR10024PA/VA-1 Neuron Rat NSE Eno2 enolase 2, gamma (Eno2), neuron-specific enolase (NSE)
SR10025PA/VA-1 Neuron Mouse Ta1 a-tubulin Tuba1a tubulin, alpha 1A (Tuba1a), Tuba-1, Tuba1
SR10026PA/VA-1 Oligodendrocyte Mouse MBP Mbp myelin basic protein (Mbp), myelin deficient; shiverer
SR10027PA/VA-1 Photoreceptor Human Opsin OPN1SW opsin 1 (cone pigments), short-wave-sensitive (color blindness, tritan)
SR10034PA/VA-1 Neural Stem Cell Rat Nestin Nes Nes
SR10035PA/VA-1 Neural Stem Cell Human Nestin NES NES
SR10041PA/VA-1 Neuron Human Doublecortin DCX DBCN, DC, LISX, SCLH, XLIS
SR10047PA/VA-1 Neuron Human MAP2 MAP2 MAP2A, MAP2B, MAP2C
SR10048PA/VA-1 Neuron Human FABP7 FABP7 B-FABP, BLBP, DKFZp547J2313, FABPB, MRG

Myogenic differentiation reporters

Catalog # Target cell type Species Promoter/enhancer element Official name Additional names
SR10010PA/VA-1 Cardiomyocyte Mouse Actc Actc1 Cardiac muscle alpha actin 1
SR10011PA/VA-1 Cardiomyocyte Human MLC-2v MYL2 MYL2, MLC2
SR10012PA/VA-1 Cardiomyocyte Human TNNT2 TNNT2 Troponin T type 2 (cardiac), TnTC, cTnT
SR10013PA/VA-1 Cardiomyocyte Mouse Tnnt2 Tnnt2 Tnt, cTnT
SR10014PA/VA-1 Smooth muscle myocyte Mouse SM22a Tagln Transgelin (Tagln), SM22, Sm22a
SR10049PA/VA-1 Cardiomyocyte Human ACTC ACTC1 ACTC, CMD1R
SR10050PA/VA-1 Skeletal myocyte Mouse Myogenin Myog myo; MYF4

Hematopoietic differentiation reporters

Catalog # Target cell type Species Promoter/enhancer element Official name Additional names
SR1004PA/VA-1 B-cell Human B29 CD79B CD79b, Ig-beta, Igb, Igbeta
SR1005PA/VA-1 B-cell Mouse B29 Cd79b Cd79b
SR1006PA/VA-1 CD8 T-cell Mouse CD8 Cd8a BB154331, Ly-2, Ly-35, Ly-B, Lyt-2
SR1007PA/VA-1 Erythroid Human HLA-DRa HLA-DRA HLA-DRA1, major histocompatibility complex, class II, DR alpha
SR1008PA/VA-1 Macrophage, microglia Mouse Cd68 Cd68 macrosialin, Scard1, gp110
SR1009PA/VA-1 Pan T-cell Human CD2 CD2 SRBC, T11
SR10032PA/VA-1 Lymphocyte Human LCK LCK YT16, p56lck, pp58lck

Structural differentiation reporters

Catalog # Target cell type Species Promoter/enhancer element Official name Additional names
SR1001PA/VA-1 Chondrocyte Mouse Col2a1 Col2a1 collagen, type II, alpha 1
SR1002PA/VA-1 Osteoblast Human SPP1 SPP1 secreted phosphoprotein 1 (SPP1), osteopontin (OPN)
SR1003PA/VA-1 Osteoblast Human Osteocalcin BGLAP bone gamma-carboxyglutamate (BGLAP), BGP, PMF1
SR10036PA/VA-1 Adipocyte Mouse ALBP Fabp4 422/aP2, ALBP/Ap2, Ap2, Lbpl
SR10038PA/VA-1 Epithelium Human Keratin 14 KRT14 CK14, EBS3, EBS4, K14, NFJ

Signaling differentiation reporters

Catalog # Target cell type Species Promoter/enhancer element Official name Additional names
SR10028PA/VA-1 Beta cell Human Insulin INS insulin
SR10037PA/VA-1 Islet Human NGN3 NGN3 neurogenin 3, Atoh5, Math4B, bHLHa7, neurog3
SR10039PA/VA-1 Islet Human PDX1 PDX1 IDX-1, IPF1, IUF1, MODY4, PDX-1, STF-1
SR10040PA/VA-1 Islet Mouse Pdx1 Pdx1 IDX-1, IPF-1, Ipf1, Mody4, STF-1, pdx-1

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Mouse α-Tubulin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch mouse neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonstmouseing the specificity of SBI’s mouse α-Tubulin pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.