pGreenZeo-mCMV Differentiation Reporter Negative Control

Run your pGreenZeo projects with confidence with the addition of this negative control that drives dscGFP and zeomycin resistance with a minimal CMV promoter

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pGreenZeo-mCMV Plasmid (negative control)

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pGreenZeo-mCMV Plasmid (negative control)

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10 µg
SR500PA-1
$ 426

pGreenZeo-mCMV Virus (negative control)

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pGreenZeo-mCMV Virus (negative control)

[variation_id] => 65636 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x 10^6 IFUs
SR500VA-1
$ 547
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Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenZeo projects—SBI’s already built one for you. The GreenZeo cassette is driven by a minimal CMV promoter with the dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin marker co-expression mediated by a T2A element. This configuration provides a control for background levels of GFP and zeomycin expression in the absence of enhancer elements, such as the ones used in our Pluripotency Reporters.

Choose the pluripotency reporter that’s right for you

Pluripotency Reporters

Catalog # Species Promoter/enhancer element Reporter
SR10033PA/VA-1 Human Oct-4 pGreenZeo
SR10043PA/VA-1 Human Oct-4 pRedZeo
SR10053PA/VA-1 Human Oct-4 pRedTK
SR10029PA/VA-1 Mouse Oct-4 pGreenZeo
SR10045PA/VA-1 Mouse Oct-4 pRedZeo
SR10054PA/VA-1 Mouse Oct-4 pRedTK
SR10030PA/VA-1 Human Nanog pGreenZeo
SR10042PA/VA-1 Human Nanog pRedZeo
SR10055PA/VA-1 Human Nanog pRedTK
SR10031PA/VA-1 Mouse Nanog pGreenZeo
SR10044PA/VA-1 Mouse Nanog pRedZeo
SR10056PA/VA-1 Mouse Nanog pRedTK

Supporting Data

See some of our differentiation reporters in action

SBI’s differentiation reporters are used in a number of papers. The data shown below are just one example (from Ravin R, Hoeppner DJ, Munno DM, Carmel L, Sullivan J, Levitt DL, Miller JL, Athaide C, Panchision DM, McKay RD. Potency and fate specification in CNS stem cell populations in vitro. Cell Stem Cell. 2008 Dec 4; 3(6):670-80. PMID: 19041783)

Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human Nestin pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonsthumaning the specificity of SBI’s human Nestin pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.