Monitor neurogenesis in real time
With SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Human GFAP pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP; 2-hour half-life) and zeomycin resistance from the human GFAP promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin. This vector also constitutively expresses a puromycin marker expressed from the EF1α promoter.
• Create stable neurogenesis-reporting cell lines
• Monitor multiple lineages simultaneously
• Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.
Choose the differentiation reporter that’s right for you
Neural differentiation reporters