Human GFAP pGreenZeo (EF1α-Puro) Differentiation Reporter

Study neurogenesis by expressing GFP in cells where the GFAP promoter/enhancer is active (with puro)

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Human GFAP Differentiation Reporter (pGreenZeo, Virus) Puro

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Human GFAP Differentiation Reporter (pGreenZeo, Virus) Puro

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>2 x 10^6 IFUs
SR10015VA-P
$ 621
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Overview

Monitor neurogenesis in real time
With SBI’s line of pGreenZeo, pRedZeo, and pRedTK Differentation Reporter Vectors, you can monitor stem cell differentiation in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built differentiation reporters come as ready-to-package lentivector plasmids or ready-to-transduce pre-packaged lentivirus. The Human GFAP pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP; 2-hour half-life) and zeomycin resistance from the human GFAP promoter/enhancer elements, enabling visualization of neurogenesis using GFP fluorescence and selection for the desired cells using zeomycin. This vector also constitutively expresses a puromycin marker expressed from the EF1α promoter.

• Create stable neurogenesis-reporting cell lines
• Monitor multiple lineages simultaneously
• Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.
Choose the differentiation reporter that’s right for you
Neural differentiation reporters
<img src=Live imaging of neuronal differentiation

Figure 1. Live imaging of neuronal differentiation. Ravin, et al, used SBI’s Human GFAP pGreenFire Differentiation Reporter (Cat.# SR10016VA-1), which drives GFP expression from the glial fibrillary acidic protein promoter, to watch human neural stem cells differentiate into a network of mature neurons, oligodendrocytes, and astrocytes over the course of seven days. The periodic “flashes” seen in this video correspond to fluorescent photos taken of the growing cells to identify the GFP signals. The final photo taken after the network formation is shown below the video (color added). Among the network of neurons, only the astrocytes are bright green, demonstrating the specificity of SBI’s human GFAP pGreenFire Differentiation Reporter.

Simultaneously track multiple lineages from iPS and progenitor cells

Figure 2. Simultaneously track multiple lineages from iPS and progenitor cells.

See SBI’s differentiation reporters in action

Figure 3. Additional differentiation reporter data.