pGreenFire1-RARE Lentivector

Study RARE signal transduction by co-expressing dscGFP and luciferase in response to RARE activity

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pGreenFire1-RARE (plasmid)
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pGreenFire1-RARE (plasmid)

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10 µg
TR037PA-1
$ 660

pGreenFire1-RARE (virus)
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pGreenFire1-RARE (virus)

[variation_id] => 65831 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x 10^6 IFUs
TR037VA-1
$ 660
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Overview

Monitor signal transduction in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-RARE Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from RARE transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of RARE-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to RARE activity. The result is the ability to quantitatively measure RARE activity by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

pGreenFire1-RARE Lentivector

To facilitate cell line construction, the pGreenFire1-RARE Lentivector also comes with a constitutively-expressed neomycin marker (EF1α-neo; Cat.# TR037PA-N) or puromycin marker (EF1α-neo; Cat.# TR037PA-P). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action

Product Documentation

Citations

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  • Zeuner, M, et al. (2017) Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation. Mediators of Inflammation. ;. Link: Mediators of Inflammation
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  • Levy, G, et al. (2016) Nuclear receptors control pro-viral and antiviral metabolic responses to hepatitis C virus infection. Nat. Chem. Biol.. 2016 Dec 1; 12(12):1037-1045. PM ID: 27723751
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  • Wang, Y, et al. (2016) 3K3A-activated protein C stimulates postischemic neuronal repair by human neural stem cells in mice. Nat. Med.. 2016 Sep 1; 22(9):1050-5. PM ID: 27548576
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