Supporting your studies with ready-to-go controls
No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.
To simplify cell line construction, this pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector comes with a constitutively expressed puromycin marker expressed from the Ef1α promoter. Or choose an EF1α-neo (Cat.# TR010PA-N), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.