pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector

Run your pGreenFire projects with confidence with the addition of this negative control that drives copGFP and luciferase with a minimal CMV promoter (EF1α-puro)

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pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)+ EF1α-Puro

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pGreenFire1-mCMV Plasmid (pTRH1 mCMV dscGFP T2A Fluc, negative control)+ EF1α-Puro

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10 µg
TR010PA-P
$ 516

pGreenFire1-mCMV Virus (pTRH1 mCMV dscGFP T2A Fluc) + EF1α-Puro

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pGreenFire1-mCMV Virus (pTRH1 mCMV dscGFP T2A Fluc) + EF1α-Puro

[variation_id] => 65686 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x 10^6 IFUs
TR010VA-P
$ 647
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Overview

Supporting your studies with ready-to-go controls

No need to make a negative control for your pGreenFire projects—SBI’s already built one for you. With the pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector, the GreenFire cassette is driven by a minimal CMV promoter with destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase co-expression mediated by a T2A element. The minimal CMV promoter delivers negligible expression, and this configuration provides a control for background levels of GFP and luciferase expression in the absence of enhancer elements, such as the ones used in our Signal Transduction Pathway Reporters/Transcriptional Response Element Reporters.

pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector

To simplify cell line construction, this pGreenFire1-mCMV (EF1α-puro) Negative Control Lentivector comes with a constitutively expressed puromycin marker expressed from the Ef1α promoter. Or choose an EF1α-neo (Cat.# TR010PA-N), or no marker at all (Cat.# TR010PA-1). All versions of this lentivector are available as plasmid or pre-packaged virus.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action


Citations

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