pGreenFire1-GAS (EF1α-neo) Lentivector

Study GAS signal transduction by co-expressing dscGFP and luciferase in response to GAS activity (includes EF1α-neo selection cassette)

Description
Size
Catalog Number
Price
Quantity
Add to Cart

pGreenFire1-GAS (plasmid)+ EF1α-Neo

Array ( [attributes] => Array ( [attribute_size] => 10 µg ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 647 [display_regular_price] => 647 [image] => Array ( [title] => pgreenfire1-gas-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 20860 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $647 [sku] => TR027PA-N [variation_description] =>

pGreenFire1-GAS (plasmid)+ EF1α-Neo

[variation_id] => 65765 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
TR027PA-N
$ 647

pGreenFire1-GAS (virus) + EF1α-Neo

Array ( [attributes] => Array ( [attribute_size] => >2 x 10^6 IFUs ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 647 [display_regular_price] => 647 [image] => Array ( [title] => pgreenfire1-gas-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/pgreenfire1-gas-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 20860 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $647 [sku] => TR027VA-N [variation_description] =>

pGreenFire1-GAS (virus) + EF1α-Neo

[variation_id] => 65769 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x 10^6 IFUs
TR027VA-N
$ 647
Contact Us Speak to a specialist
1-888-266-5066

Overview

Monitor signal transduction in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-GAS (EF1α-neo) Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from GAS transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of GAS-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to GAS activity. The result is the ability to quantitatively measure GAS activity by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

pGreenFire1-GAS (EF1α-neo) Lentivector

To facilitate cell line construction, the pGreenFire1-GAS (EF1α-neo) Lentivector comes with a constitutively-expressed neomycin marker from the EF1α promoter. Or you can choose a puromycin marker (EF1α-neo; Cat.# TR027PA-P) or no marker at all (Cat.# TR027PA-1). In addition, all forms of this vector are available as both lentivector plasmid, and pre-packaged virus.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cellsTrack and measure the activity of oncogenic signal transduction pathways in live cells

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesisDevelop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

General pGreenFire data examplesSee SBI’s pGreenFire1 reporters in action

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in actionSee SBI’s pGreenFire1 reporters in action


Citations

  • Heun, Y, et al. (2017) HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2. Mol. Ther.. 2017 Jul 5; 25(7):1616-1627. PM ID: 28434868
  • Phua, KKL, Liu, Y & Sim, SH. (2017) Non-linear enhancement of mRNA delivery efficiencies by influenza A derived NS1 protein engendering host gene inhibition property. Biomaterials. 2017 Jul 1; 133:29-36. PM ID: 28426973
  • Takada, S, et al. (2017) Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J. Allergy Clin. Immunol.. 2017 Jun 3;. PM ID: 28587749
  • Li, X, et al. (2017) Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol. Cell. 2017 Jun 1; 66(5):684-697.e9. PM ID: 28552616
  • Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017 May 23; 11(5):4660-4668. PM ID: 28480715
  • Choi, EJ, et al. (2017) A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017 May 15;. PM ID: 28504720
  • Krabbe, G, et al. (2017) Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc. Natl. Acad. Sci. U.S.A.. 2017 May 9; 114(19):5029-5034. PM ID: 28438992
  • Schmidt, C, et al. (2017) Pre-clinical drug screen reveals topotecan, actinomycin D and volasertib as potential new therapeutic candidates for ETMR brain tumor patients. Neuro-oncology. 2017 May 8;. PM ID: 28482026
  • Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017 May 2; 8(3). PM ID: 28465426
  • Gryder, BE, et al. (2017) PAX3-FOXO1 Establishes Myogenic Super Enhancers and Confers BET Bromodomain Vulnerability. Cancer Discov. 2017 Apr 26;. PM ID: 28446439
  • Jin, C, et al. (2017) Over-expression of ASIC1a promotes proliferation via activation of the β-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget. 2017 Apr 18; 8(16):25977-25988. PM ID: 27462920
  • Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res.. 2017 Apr 1; 140:1-12. PM ID: 28077314
  • Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1;:2472555216689772. PM ID: 28346099
  • Gujar, AD, et al. (2016) An NAD+-dependent transcriptional program governs self-renewal and radiation resistance in glioblastoma. Proc. Natl. Acad. Sci. U.S.A.. 2016 Dec 20; 113(51):E8247-E8256. PM ID: 27930300
  • Levy, G, et al. (2016) Nuclear receptors control pro-viral and antiviral metabolic responses to hepatitis C virus infection. Nat. Chem. Biol.. 2016 Dec 1; 12(12):1037-1045. PM ID: 27723751
  • Ha, SJ, et al. (2016) Preventive effect of Rhus javanica extract on UVB-induced skin inflammation and photoaging. Journal of Functional Foods. 2016 Dec 1; 27:589-599. Link: Journal of Functional Foods
  • Verma, RK, et al. (2016) α-Mangostin-encapsulated PLGA nanoparticles inhibit pancreatic carcinogenesis by targeting cancer stem cells in human, and transgenic (Kras(G12D), and Kras(G12D)/tp53R270H) mice. Sci Rep. 2016 Sep 14; 6:32743. PM ID: 27624879
  • Wang, Y, et al. (2016) 3K3A-activated protein C stimulates postischemic neuronal repair by human neural stem cells in mice. Nat. Med.. 2016 Sep 1; 22(9):1050-5. PM ID: 27548576
  • Sarett, SM, et al. (2016) Hydrophobic interactions between polymeric carrier and palmitic acid-conjugated siRNA improve PEGylated polyplex stability and enhance in vivo pharmacokinetics and tumor gene silencing. Biomaterials. 2016 Aug 1; 97:122-32. PM ID: 27163624
  • Zhang, Z, et al. (2016) Microfluidics 3D gel-island chip for single cell isolation and lineage-dependent drug responses study. Lab Chip. 2016 Jul 7; 16(13):2504-12. PM ID: 27270563