pGF-EGR1-mCMV-EF1α-Puro Lentivector

Study oncogenic signaling by co-expressing dscGFP and luciferase in response to EGR1 activity at EGR1 transcriptional response elements

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pGF-EGR1-mCMV-EF1α-Puro (293 stable cell line)

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pGF-EGR1-mCMV-EF1α-Puro (293 stable cell line)

[variation_id] => 65911 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
2 x 10^6 Cells
TR204a-p
$ 2826

pGF-EGR1-mCMV-EF1α-Puro (plasmid)

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pGF-EGR1-mCMV-EF1α-Puro (plasmid)

[variation_id] => 65912 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
TR204pa-p
$ 647

pGF-EGR1-mCMV-EF1α-Puro (virus)

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pGF-EGR1-mCMV-EF1α-Puro (virus)

[variation_id] => 65913 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x 10^6 IFUs
TR204va-p
$ 647
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Overview

Monitor oncogenic signaling in real time

With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid*, ready-to-transduce pre-packaged lentivirus, and as a ready-to-study cell line. The pGF-EGR1-mCMV-EF1α-Puro Lentivector co-expresses a destabilized copepod GFP (dscGFP; 2-hour half-life) and luciferase from EGR1 transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of EGR1 TREs, drives expression of dscGFP and luciferase in response to EGR1 activity. The result is the ability to quantitatively measure EGR1 activity at EGR1 TREs by fluorescence and luciferase activity.

  • Sort responsive cells with dscGFP
  • Measure activity with luciferase
  • Leverage SBI’s highly-regarded lentivectors
  • Create stable signaling pathway reporter cell lines
  • Introduce reporters into difficult-to-transfect cell types, including primary and non-dividing mammalian cell lines

pGF-EGR1-mCMV-EF1α-Puro LentivectorThe pGF-EGR1-mCMV-EF1α-Puro Lentivector is available as a lentivector, pre-packaged virus, and a stable cell line created from the pGF-EGR1-mCMV-EF1α-Puro Lentivector transduced into 293 cells.

*Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Supporting Data

See our transcriptional response element reporters in action

Monitor oncogenic pathway reporters

Track and measure the activity of oncogenic signal transduction pathways in live cells

Track and measure the activity of oncogenic signal transduction pathways in live cells

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

Develop target gene-specific LXR agonists that could regulate reverse cholesterol transport without increasing lipogenesis

 

General pGreenFire data examples

See SBI’s pGreenFire1 reporters in action

 

Monitoring NF-κB transactivationSee SBI’s pGreenFire1 reporters in action

 

See SBI’s pGreenFire1 reporters in action


Citations

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  • Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017 May 23; 11(5):4660-4668. PM ID: 28480715
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  • Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res.. 2017 Apr 1; 140:1-12. PM ID: 28077314
  • Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1;:2472555216689772. PM ID: 28346099
  • Gujar, AD, et al. (2016) An NAD+-dependent transcriptional program governs self-renewal and radiation resistance in glioblastoma. Proc. Natl. Acad. Sci. U.S.A.. 2016 Dec 20; 113(51):E8247-E8256. PM ID: 27930300
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