NF-κB/Jurkat/GFPTM Transcriptional Reporter Cell Line

Monitor NF-κB signaling in vitro in a physiologically-relevant cell line to screen for genetic and/or small molecule inhibitors and activators, and more

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NF-kB/Jurkat/GFP Transcriptional Reporter Cell Line

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NF-kB/Jurkat/GFP Transcriptional Reporter Cell Line

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2 x 10^6 Cells
$ 3336
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Monitor NF-κB signaling in Jurkat cells

Speed your studies with this pre-built Jurkat cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP cassette. Expression of GFP (up to 30-fold over background) only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway.

  • Study NF-κB signaling in physiologically-relevant Jurkat cells
  • Use for fluorescence microscopy or FACS
  • Take advantage of the cell line’s 30-fold over background NF-κB-dependent GFP expression
  • Compatible with a variety of screening methods including small molecule and RNAi

Supporting Data

See the NF-κB/Jurkat/GFP Transcriptional Reporter Cell Line in action

Strong GFP fluorescence in response to signaling enables FACS

Strong GFP fluorescence in response to signaling enables FACS

The NF-κB/Jurkat/GFP provides a strong, dose-dependent response to TNF-αThe NF-κB/Jurkat/GFP provides a strong, dose-dependent response to TNF-α

METHODS: NF-B/Jurkat/GFP™ Reporter cells (5×105 cells) were plated at a concentration of 1 million cells/mL into each well of a 24-well plate. TNF-α was added in the amount indicated in the figure. After 24 hours, GFP fluorescence was measured and intensities plotted against TNF-α concentration (A). The fluorescent cells in the original 24-well plate were also photographed on a Zeiss inverted epi-fluorescence microscope (B). Alternatively, 200 μL of cells were fixed with formaldehyde and GFP reporter induction analyzed by flow cytometry, and either the GFP intensities (C) or the percentage of GFP positive cells (D) were plotted against the amount of TNF-α.

The NF-κB/Jurkat/GFP provides appropriate T cell receptor-mediated signalingThe NF-κB/Jurkat/GFP provides appropriate T cell receptor-mediated signaling


  • Heun, Y, et al. (2017) HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2. Mol. Ther.. 2017 Jul 5; 25(7):1616-1627. PM ID: 28434868
  • Phua, KKL, Liu, Y & Sim, SH. (2017) Non-linear enhancement of mRNA delivery efficiencies by influenza A derived NS1 protein engendering host gene inhibition property. Biomaterials. 2017 Jul 1; 133:29-36. PM ID: 28426973
  • Takada, S, et al. (2017) Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J. Allergy Clin. Immunol.. 2017 Jun 3;. PM ID: 28587749
  • Li, X, et al. (2017) Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol. Cell. 2017 Jun 1; 66(5):684-697.e9. PM ID: 28552616
  • Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017 May 23; 11(5):4660-4668. PM ID: 28480715
  • Choi, EJ, et al. (2017) A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017 May 15;. PM ID: 28504720
  • Krabbe, G, et al. (2017) Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc. Natl. Acad. Sci. U.S.A.. 2017 May 9; 114(19):5029-5034. PM ID: 28438992
  • Schmidt, C, et al. (2017) Pre-clinical drug screen reveals topotecan, actinomycin D and volasertib as potential new therapeutic candidates for ETMR brain tumor patients. Neuro-oncology. 2017 May 8;. PM ID: 28482026
  • Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017 May 2; 8(3). PM ID: 28465426
  • Gryder, BE, et al. (2017) PAX3-FOXO1 Establishes Myogenic Super Enhancers and Confers BET Bromodomain Vulnerability. Cancer Discov. 2017 Apr 26;. PM ID: 28446439
  • Jin, C, et al. (2017) Over-expression of ASIC1a promotes proliferation via activation of the β-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget. 2017 Apr 18; 8(16):25977-25988. PM ID: 27462920
  • Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res.. 2017 Apr 1; 140:1-12. PM ID: 28077314
  • Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1;:2472555216689772. PM ID: 28346099
  • Gujar, AD, et al. (2016) An NAD+-dependent transcriptional program governs self-renewal and radiation resistance in glioblastoma. Proc. Natl. Acad. Sci. U.S.A.. 2016 Dec 20; 113(51):E8247-E8256. PM ID: 27930300
  • Levy, G, et al. (2016) Nuclear receptors control pro-viral and antiviral metabolic responses to hepatitis C virus infection. Nat. Chem. Biol.. 2016 Dec 1; 12(12):1037-1045. PM ID: 27723751
  • Ha, SJ, et al. (2016) Preventive effect of Rhus javanica extract on UVB-induced skin inflammation and photoaging. Journal of Functional Foods. 2016 Dec 1; 27:589-599. Link: Journal of Functional Foods
  • Verma, RK, et al. (2016) α-Mangostin-encapsulated PLGA nanoparticles inhibit pancreatic carcinogenesis by targeting cancer stem cells in human, and transgenic (Kras(G12D), and Kras(G12D)/tp53R270H) mice. Sci Rep. 2016 Sep 14; 6:32743. PM ID: 27624879
  • Wang, Y, et al. (2016) 3K3A-activated protein C stimulates postischemic neuronal repair by human neural stem cells in mice. Nat. Med.. 2016 Sep 1; 22(9):1050-5. PM ID: 27548576
  • Sarett, SM, et al. (2016) Hydrophobic interactions between polymeric carrier and palmitic acid-conjugated siRNA improve PEGylated polyplex stability and enhance in vivo pharmacokinetics and tumor gene silencing. Biomaterials. 2016 Aug 1; 97:122-32. PM ID: 27163624
  • Zhang, Z, et al. (2016) Microfluidics 3D gel-island chip for single cell isolation and lineage-dependent drug responses study. Lab Chip. 2016 Jul 7; 16(13):2504-12. PM ID: 27270563