NF-κB/293/GFP-LucTM Transcriptional Reporter Cell Line

Monitor NF-κB signaling in vitro and screen for genetic and/or small molecule inhibitors and activators, and more – sort with GFP and quantify with luciferase

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NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line

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NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line

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2 x 10^6 Cells
TR860A-1
$ 2826
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Overview

Monitor NF-κB signaling in Jurkat cells

Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP-luciferase cassette. Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway.

  • Study NF-κB signaling in 293 TN cells
  • Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
  • Measure pathway activity with luciferase
  • Compatible with a variety of screening methods including small molecule and RNAi

Supporting Data

See the NF-κB/293/GFP-luc Transcriptional Reporter Cell Line in action

Monitor NF-κB in real time with GFP

Monitor NF-κB in real time with GFP

Quantitate NF-κB Activation with luciferase assaysQuantitate NF-κB Activation with luciferase assays


Citations

  • Heun, Y, et al. (2017) HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2. Mol. Ther.. 2017 Jul 5; 25(7):1616-1627. PM ID: 28434868
  • Phua, KKL, Liu, Y & Sim, SH. (2017) Non-linear enhancement of mRNA delivery efficiencies by influenza A derived NS1 protein engendering host gene inhibition property. Biomaterials. 2017 Jul 1; 133:29-36. PM ID: 28426973
  • Takada, S, et al. (2017) Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J. Allergy Clin. Immunol.. 2017 Jun 3;. PM ID: 28587749
  • Li, X, et al. (2017) Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol. Cell. 2017 Jun 1; 66(5):684-697.e9. PM ID: 28552616
  • Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano. 2017 May 23; 11(5):4660-4668. PM ID: 28480715
  • Choi, EJ, et al. (2017) A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene. 2017 May 15;. PM ID: 28504720
  • Krabbe, G, et al. (2017) Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc. Natl. Acad. Sci. U.S.A.. 2017 May 9; 114(19):5029-5034. PM ID: 28438992
  • Schmidt, C, et al. (2017) Pre-clinical drug screen reveals topotecan, actinomycin D and volasertib as potential new therapeutic candidates for ETMR brain tumor patients. Neuro-oncology. 2017 May 8;. PM ID: 28482026
  • Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio. 2017 May 2; 8(3). PM ID: 28465426
  • Gryder, BE, et al. (2017) PAX3-FOXO1 Establishes Myogenic Super Enhancers and Confers BET Bromodomain Vulnerability. Cancer Discov. 2017 Apr 26;. PM ID: 28446439
  • Jin, C, et al. (2017) Over-expression of ASIC1a promotes proliferation via activation of the β-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget. 2017 Apr 18; 8(16):25977-25988. PM ID: 27462920
  • Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res.. 2017 Apr 1; 140:1-12. PM ID: 28077314
  • Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov. 2017 Jan 1;:2472555216689772. PM ID: 28346099
  • Gujar, AD, et al. (2016) An NAD+-dependent transcriptional program governs self-renewal and radiation resistance in glioblastoma. Proc. Natl. Acad. Sci. U.S.A.. 2016 Dec 20; 113(51):E8247-E8256. PM ID: 27930300
  • Levy, G, et al. (2016) Nuclear receptors control pro-viral and antiviral metabolic responses to hepatitis C virus infection. Nat. Chem. Biol.. 2016 Dec 1; 12(12):1037-1045. PM ID: 27723751
  • Ha, SJ, et al. (2016) Preventive effect of Rhus javanica extract on UVB-induced skin inflammation and photoaging. Journal of Functional Foods. 2016 Dec 1; 27:589-599. Link: Journal of Functional Foods
  • Verma, RK, et al. (2016) α-Mangostin-encapsulated PLGA nanoparticles inhibit pancreatic carcinogenesis by targeting cancer stem cells in human, and transgenic (Kras(G12D), and Kras(G12D)/tp53R270H) mice. Sci Rep. 2016 Sep 14; 6:32743. PM ID: 27624879
  • Wang, Y, et al. (2016) 3K3A-activated protein C stimulates postischemic neuronal repair by human neural stem cells in mice. Nat. Med.. 2016 Sep 1; 22(9):1050-5. PM ID: 27548576
  • Sarett, SM, et al. (2016) Hydrophobic interactions between polymeric carrier and palmitic acid-conjugated siRNA improve PEGylated polyplex stability and enhance in vivo pharmacokinetics and tumor gene silencing. Biomaterials. 2016 Aug 1; 97:122-32. PM ID: 27163624
  • Zhang, Z, et al. (2016) Microfluidics 3D gel-island chip for single cell isolation and lineage-dependent drug responses study. Lab Chip. 2016 Jul 7; 16(13):2504-12. PM ID: 27270563