Cytosol Cyto-TracerTM, pCT-Cyto-GFPLuc (MSCV)

Study cellular processes and physiology with this Cyto-Tracer that localizes copGFP and luciferase to the cytosol – reporter expression is driven by MSCV

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pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged)

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pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged)

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10 µg
CYTO121-PB-1
$ 558

pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged; pre-packaged virus)

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pCT-Cyto-GFPLuc (pMSCV, Cytosol, GFP+Luciferase Untagged; pre-packaged virus)

[variation_id] => 63616 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
CYTO121-VB-1
$ 644
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Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-TracersTM
Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-GFPLuc (MSCV), co-expresses an untagged copGFP and luciferase, both of which naturally localize to the cytosol. copGFP and luciferase are co-expressed from the MSCV promoter for strong expression in hematopoietic and stem cells.

· Stable lentivector-based system
· Great for creating stable reporter cell lines
· Ideal for co-localization studies
· Monitor cellular dynamics and functional studies in real time
· Label cells with GFP for a broad range of studies
Choose the Cyto-Tracer that’s right for you

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               <h2>How It Works</h2>
               <p>Visualizing the cell with Cyto-Tracers<br />
<p>
SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.<br />
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Supporting Data

See some of our Cyto-Tracers in action

SBI’s Cyto-Tracers in action


Citations

  • Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567
  • Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017 Jun 1; 38(6):592-603. PM ID: 28402394
  • Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368
  • Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016 Jan 1; 37(1):313-21. PM ID: 26211004
  • Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015 Jun 24; 6:7506. PM ID: 26103923
  • Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014 Nov 26; 5:5513. PM ID: 25423885
  • Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472
  • Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014 Nov 4; 5:5266. PM ID: 25366663
  • Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. ;. Link: Thesis
  • Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. ;. Link: Thesis
  • Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. ;. Link: Thesis
  • VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. ;. Link: Thesis