Cytosol Cyto-TracerTM, pCT-Cyto-GFP (CMV)

Study cellular processes and physiology with this Cyto-Tracer that expresses un-tagged copGFP from the CMV promoter - untagged copGFP localizes in the cytosol

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pCT-Cyto-GFP (pCMV, Cytosol, Untagged)

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pCT-Cyto-GFP (pCMV, Cytosol, Untagged)

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10 µg
CYTO118-PA-1
$ 558

pCT-Cyto-GFP (pCMV, Cytosol, Untagged; pre-packaged virus)

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pCT-Cyto-GFP (pCMV, Cytosol, Untagged; pre-packaged virus)

[variation_id] => 63603 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2x10^6 IFUs
CYTO118-VA-1
$ 644
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Overview

Easily study the dynamics of cellular processes with SBI’s Cyto-TracersTM

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The Cytosol Cyto-Tracer, pCT-Cyto-GFP (CMV), expresses an untagged copGFP, which naturally localizes to the cytosol. copGFP is expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.

  • Stable lentivector-based system
  • Great for creating stable reporter cell lines
  • Ideal for co-localization studies
  • Monitor cellular dynamics and functional studies in real time
  • Label cells with GFP for a broad range of studies

Choose the Cyto-Tracer that’s right for you

Promoter (Cat.#) Cyto-Tracer Target Location Peptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFP Plasma Membrane Neuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFP Inner Leaflet of Membrane Farnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFP Mitochondria Cytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1) pCT-ER-GFP Endoplasmic Reticulum ER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1) pCT-Golgi-GFP Golgi Apparatus Galactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1) pCT-Secretory-GFP ER-Golgi  Vesicles Secretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1) pCT-Lyso-GFP Lysosome Cathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1) pCT-Endo-GFP Endosome RhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1) pCT-Endo-RFP Endosome RhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFP Peroxisome Peroxisomal consensus signal
CMV (CYTO120-PA-1/CYTO120-VA-1) pCT-CD63-GFP Autophagosome LC3
CMV (CYTO120R-PA-1/CYTO120R-VA-1) pCT-CD63-RFP Autophagosome LC3
CMV (CYTO109-PA-1/CYTO109-VA-1) pCT-Autophago-GFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO109R-PA-1/CYTO109R-VA-1) pCT-Autophago-RFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1) pCT-CD9-GFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1) pCT-CD9-RFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1) pCT-CD81-GFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1) pCT-CD81-RFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFP Cytoskeleton β-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFP Cytoskeleton α-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1) pCT-Tublin-RFP Cytoskeleton α-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1) pCT-MAP4-GFP Microtubule Microtubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFP Nucleus H2B
CMV (CYTO114-PA-1/CYTO114-VA-1) pCT-Apoptosis-Luc  Caspase 3/7 activation Cyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1) pCT-GFP-BAX Cytosol to Mitochondria BAX
CMV (CYTO116-PA-1/CYTO116-VA-1) pCT- Catenin-GFP Cytosol to Nucleus/Cell Membrane β-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1) pCT-CMV-PSD95-GFP Dendrite Membranes PSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFP Cytosolic Compartment GFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1) pCT-Cyto-RFP Cytosolic Compartment RFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1) pCT-Cyto-GFpLuc GFP and Luciferase Control GFP and Luciferase (Untagged)

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location

Supporting Data

See some of our Cyto-Tracers in action

SBI’s Cyto-Tracers in action


Citations

  • Gonzalez-King, H, et al. (2017) Hypoxia Inducible Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes. Stem Cells. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567
  • Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival. Carcinogenesis. 2017 Jun 1; 38(6):592-603. PM ID: 28402394
  • Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase. J. Biol. Chem.. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368
  • Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes. Tumour Biol.. 2016 Jan 1; 37(1):313-21. PM ID: 26211004
  • Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun. 2015 Jun 24; 6:7506. PM ID: 26103923
  • Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β. Nat Commun. 2014 Nov 26; 5:5513. PM ID: 25423885
  • Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. J. Biol. Chem.. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472
  • Sato, Y, et al. (2014) MTCL1 crosslinks and stabilizes non-centrosomal microtubules on the Golgi membrane. Nat Commun. 2014 Nov 4; 5:5266. PM ID: 25366663
  • Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular. Thesis. ;. Link: Thesis
  • Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway. Thesis. ;. Link: Thesis
  • Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie. Thesis. ;. Link: Thesis
  • VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI. Thesis. ;. Link: Thesis