N-term Clone-your-own Cyto-TracerTM, pCT-CMV-MCS-GFP-EF1α-Puro

Create your own Cyto-Tracer for subcellular localization studies – use this vector to fuse a protein to the N-terminal end of copGFP

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pCT-CMV-MCS-GFP-EF1α-Puro (for adding fusion proteins at the N-terminus of GFP)

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pCT-CMV-MCS-GFP-EF1α-Puro (for adding fusion proteins at the N-terminus of GFP)

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10 µg
$ 558
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Easily study the dynamics of cellular processes with SBI’s Cyto-TracersTM

Leverage SBI’s high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors—available as transfectable plasmids or ready-to-transduce, pre-packaged virus. With the N-term Clone-your-own Cyto-Tracer, pCT-CMV-MCS-GFP-EF1α-Puro, you can create your own Cyto-Tracer by fusing a protein tag to the N-terminal end of copGFP. Your custom Cyto-Tracer will be expressed from the CMV promoter for strong expression in common cell types such as HeLa, HEK293, and HT1080.

  • Stable lentivector-based system
  • Great for creating stable reporter cell lines
  • Ideal for co-localization studies
  • Monitor cellular dynamics and functional studies in real time
  • Create your own subcellular label for a broad range of studies

Choose the Cyto-Tracer that’s right for you

Promoter (Cat.#) Cyto-Tracer Target Location Peptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFP Plasma Membrane Neuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFP Inner Leaflet of Membrane Farnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFP Mitochondria Cytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1) pCT-ER-GFP Endoplasmic Reticulum ER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1) pCT-Golgi-GFP Golgi Apparatus Galactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1) pCT-Secretory-GFP ER-Golgi  Vesicles Secretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1) pCT-Lyso-GFP Lysosome Cathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1) pCT-Endo-GFP Endosome RhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1) pCT-Endo-RFP Endosome RhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFP Peroxisome Peroxisomal consensus signal
CMV (CYTO120-PA-1/CYTO120-VA-1) pCT-CD63-GFP Autophagosome LC3
CMV (CYTO120R-PA-1/CYTO120R-VA-1) pCT-CD63-RFP Autophagosome LC3
CMV (CYTO109-PA-1/CYTO109-VA-1) pCT-Autophago-GFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO109R-PA-1/CYTO109R-VA-1) pCT-Autophago-RFP Exosome/Secretory CD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1) pCT-CD9-GFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1) pCT-CD9-RFP Exosome/Secretory CD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1) pCT-CD81-GFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1) pCT-CD81-RFP Exosome/Secretory CD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFP Cytoskeleton β-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFP Cytoskeleton α-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1) pCT-Tublin-RFP Cytoskeleton α-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1) pCT-MAP4-GFP Microtubule Microtubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFP Nucleus H2B
CMV (CYTO114-PA-1/CYTO114-VA-1) pCT-Apoptosis-Luc  Caspase 3/7 activation Cyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1) pCT-GFP-BAX Cytosol to Mitochondria BAX
CMV (CYTO116-PA-1/CYTO116-VA-1) pCT- Catenin-GFP Cytosol to Nucleus/Cell Membrane β-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1) pCT-CMV-PSD95-GFP Dendrite Membranes PSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFP Cytosolic Compartment GFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1) pCT-Cyto-RFP Cytosolic Compartment RFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1) pCT-Cyto-GFpLuc GFP and Luciferase Control GFP and Luciferase (Untagged)

How It Works

Visualizing the cell with Cyto-Tracers

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location.

SBI’s Cyto-Tracers fuse a specific protein tag to copGFP or RFP, directing the fluorescent protein to the desired subcellular location

Supporting Data

See some of our Cyto-Tracers in action

SBI’s Cyto-Tracers in action


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