MSCV-Luciferase-EF1α-copGFP-T2A-Puro BLIV 2.0 Lentivector for In Vivo Imaging

Use this BLIV 2.0 vector to track cell fate after implantation, or other in vivo imaging applications – MSCV drives luciferase and EF1α drives copGFP and puro

Description
Size
Catalog Number
Price
Quantity
Add to Cart

BLIV 2.0 Reporter: MSCV-Luciferase-EF1α-copGFP-T2A-Puro Lentivector Plasmid

Array ( [attributes] => Array ( [attribute_size] => 10 µg ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 663 [display_regular_price] => 663 [image] => Array ( [title] => bliv-main-image-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 20856 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $663 [sku] => BLIV713PA-1 [variation_description] =>

BLIV 2.0 Reporter: MSCV-Luciferase-EF1α-copGFP-T2A-Puro Lentivector Plasmid

[variation_id] => 63263 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
BLIV713PA-1
$ 663

BLIV 2.0 Reporter: MSCV-Luciferase-EF1α-copGFP-T2A-Puro Pre-packaged Virus

Array ( [attributes] => Array ( [attribute_size] => >2 x10^6 IFUs ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 663 [display_regular_price] => 663 [image] => Array ( [title] => bliv-main-image-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/imaging-reporter-vectors/bliv-main-image-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 20856 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $663 [sku] => BLIV713VA-1 [variation_description] =>

BLIV 2.0 Reporter: MSCV-Luciferase-EF1α-copGFP-T2A-Puro Pre-packaged Virus

[variation_id] => 63264 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
>2 x10^6 IFUs
BLIV713VA-1
$ 663
Contact Us Speak to a specialist
1-888-266-5066

Overview

Clearly visualize cells in vivo and ex vivo for tracking cell fate and more

Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.

SBI’s lentivector-based imaging systems offer safe, stable, and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models.

MSCV-Luciferase-EF1α-copGFP-T2A-Puro BLIV 2.0 Lentivector for In Vivo Imaging

The MSCV-Luciferase-EF1α-copGFP-T2A-Puro BLIV 2.0 Lentivector for In Vivo Imaging leverages SBI’s well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. The 2.0 BLIVs have been further engineered to provide enhanced performance and reliability than the first-generation vectors.

Note that lentivectors can be used to generate stable reporter cell lines as they integrate into the genome.

The MSCV-Luciferase-EF1α-copGFP-T2A-Puro vector expresses luciferase from a CMV promoter for high expression in most cell types, and the bright copGFP from the EF1α promoter for medium expression. Available as either a plasmid or pre-packaged virus.

Which BLIV construct should you choose?

With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:

Choose a lentivector when:

  • You’re working with difficult-to-transfect cells
  • You’d like to create stable reporter cell lines
  • You’d like to integrate your reporter into the genome

Choose a minicircle vector* when you’re working with transfectable cells and:

  • You’d like episomal expression
  • You’d like to avoid introducing foreign sequences

Choose your promoter using the following table:

Promoter Expression Level Application
CMV High Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCV High Hematopoietic and stem cells
EF1α Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
UbC Low Most cell types including primary cells and stem cells

*Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.

Supporting Data

See SBI’s BLIV Vectors in action

SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection

Figure 1. Examples of how SBI’s BLIV lentivectors deliver strong luciferase activity after both transduction and transfection.

Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models

Figure 2. Examples of how SBI’s BLIV minicircle vectors deliver strong luciferase activity in small animal models.

Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity

Figure 3. Examples of how SBI’s BLIV lentivectors and minicircle vectors can be used to generate stable cell lines with strong luciferase activity.