Clearly visualize cells in vivo and ex vivo for tracking cell fate and more
Easily label cell lines for tracking cell fate after implantation in an animal model or other imaging applications with SBI’s line of Bioluminescent, Fluorescent, and PET Imaging Vectors (BLIV). Available in a variety of promoter-reporter combinations that enable multiple imaging modalities—fluorescence with copGFP, bioluminescence with luciferase, and positron emission tomography (PET) with thymidine kinase (TK)—you can select from lentivector or minicircle technologies for both integrated and episomal expression.
SBI’s lentivector-based imaging systems offer safe, stable, and effective gene delivery in virtually all mammalian cells in tissue culture and in whole animal models.
The CMV-GFP-T2A-Luciferase Lentivector for In Vivo Imaging leverages SBI’s well-regarded lentivector technology for either transduction or transfection of the reporter into target cells. Because the vector becomes integrated into the genome, you can use this vector to create stable reporter cell lines.
The vector co-expresses copGFP and luciferase from a CMV promoter for strong expression in most common cell types, such as HeLa, HEK293, or HT1080, with co-expression mediated by a T2A element. Available as either a plasmid or pre-packaged virus.
Which BLIV construct should you choose?
With a range of options, SBI’s vectors for in vivo imaging support a wide range of projects. Simply choose the vector that best fits your needs:
Choose a lentivector when:
- You’re working with difficult-to-transfect cells
- You’d like to create stable reporter cell lines
- You’d like to integrate your reporter into the genome
Choose a minicircle vector* when you’re working with transfectable cells and:
- You’d like episomal expression
- You’d like to avoid introducing foreign sequences
Choose your promoter using the following table:
|CMV||High||Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.)|
|MSCV||High||Hematopoietic and stem cells|
|EF1α||Medium||Most cell types including primary cells and stem cells|
|PGK||Medium||Most cell types including primary cells and stem cells|
|UbC||Low||Most cell types including primary cells and stem cells|
*Note that Minicircle Parental Vectors are only available to academic/non-profit customers. Researchers at commercial organization may only purchase the pre-made minicircle BLIVs.