PiggyBac qPCR Copy Number Kit

Find out how many copies of your PiggyBac insert have been integrated into the genome with this accurate, qPCR-based kit

Catalog Number
Add to Cart

piggyBac qPCR copy number kit, 20 reactions  

Array ( [attributes] => Array ( [attribute_size] => 20 Reactions ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 248 [display_regular_price] => 248 [image] => Array ( [title] => piggybac-copy-number-kit-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/piggybac-copy-number-kit-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 11007 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $248 [sku] => PBC100A-1 [variation_description] =>

piggyBac qPCR copy number kit, 20 reactions  

[variation_id] => 64602 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
20 Reactions
$ 248
Contact Us Speak to a specialist


Confidently count your PiggyBac inserts

When you need to know how many PiggyBac integration events have happened, turn to SBI’s PiggyBac qPCR Copy Number Kit. This kit uses qPCR to measure the number of PiggyBac inserts relative to a specific genomic locus—the UCR1 element—with the PiggyBac insert copy number calculated using the cycle threshold (Ct) values of the UCR1 signal relative to the PiggyBac insert signal.

The PiggyBac qPCR Copy Number Kit comes with enough reagents—UCR1 primers, PiggyBac primers, and cell lysis buffer—for twenty copy number determinations, and is compatible with all of SBI’s PiggyBac Vectors.

NOTE: Your cells must be passaged at least once before performing this copy number measurement to ensure that residual, non-integrated piggyBac transposon plasmid does not interfere with the qPCR reaction.

How It Works

Calculate PiggyBac insert copy number

The PiggyBac qPCR Copy Number Kit provides robust PiggyBac insert copy number determination. To calculate the PiggyBac insert copy number from the Ct values:

  1. Calculate the average Cts for the PiggyBac inserts and for the UCR1 loci (there are two UCR1 elements per genome)
  2. The copy number is the ΔΔCt/2—the ΔΔCt value must be divided by two to account for the two UCR1 elements:

ΔΔCt = 2-((average PiggyBac insert Ct) – (average UCR1 Ct))
PiggyBac insert copy number = ΔΔCt/2

You can change the number of PiggyBac insertions by adjusting the ratio of PiggyBac Vector to Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1).

Supporting Data

Robust PiggyBac insert copy number determination

Example PiggyBac qPCR Copy Number Kit data from a PiggyBac Vector titration study—the PiggyBac insert copy number can be changed by adjusting the ratio of PiggyBac Vector to Super PiggyBac Transposase Expression Vector:

[table “” not found /]

Adjust the PiggyBac insert copy number by changing the ratio of PiggyBac Vector to Super PiggyBac Transposase

Figure 1. Adjust the PiggyBac insert copy number by changing the ratio of PiggyBac Vector to Super PiggyBac Transposase. To achieve the indicated ratio of PiggyBac Vector to Super PiggyBac Transposase Expression Vector, amount of PiggyBac Vector added was 100 ng, 300 ng, 500 ng, 700 ng, and 1,000 ng, respectively, while the amount of Super PiggyBac Transposase Expression Vector was held constant at 100 ng.


  • Shinmura, K, et al. (2017) WDR62 overexpression is associated with a poor prognosis in patients with lung adenocarcinoma. Mol. Carcinog.. 2017 Aug 1; 56(8):1984-1991. PM ID: 28277612
  • Henssen, AG, et al. (2017) PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat. Genet.. 2017 Jul 1; 49(7):1005-1014. PM ID: 28504702
  • Katayama, M, et al. (2017) Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. J. Reprod. Dev.. 2017 Jun 21; 63(3):311-318. PM ID: 28331164
  • Cammareri, P, et al. (2017) TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis. Cell Death Differ.. 2017 Jun 16;. PM ID: 28622298
  • Kimura, T, et al. (2017) Hsp90 inhibitor geldanamycin attenuates the cytotoxicity of sunitinib in cardiomyocytes via inhibition of the autophagy pathway. Toxicol. Appl. Pharmacol.. 2017 Jun 15; 329:282-292. PM ID: 28624441
  • Maegawa, KI, et al. (2017) The Highly Dynamic Nature of ERdj5 Is Key to Efficient Elimination of Aberrant Protein Oligomers through ER-Associated Degradation. Structure. 2017 Jun 6; 25(6):846-857.e4. PM ID: 28479060
  • Liu, H, et al. (2017) Stochastic Protein Labeling Enables Long-Term Single Molecule Observation in vivo. bioRxiv. 2017 Jun 5;. Link: bioRxiv
  • Park, TS, Kim, SW & Lee, JH. (2017) Efficient transgene expression system using a cumate-inducible promoter and Cre-loxP recombination in avian cells. Asian-australas. J. Anim. Sci.. 2017 Jun 1; 30(6):886-892. PM ID: 27764912
  • Lin, W & Li, Z. (2017) Blueberries inhibit cyclooxygenase-1 and cyclooxygenase-2 activity in human epithelial ovarian cancer. Oncol Lett. 2017 Jun 1; 13(6):4897-4904. PM ID: 28599493
  • Yamazaki, T, et al. (2017) Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase. PLoS ONE. 2017 May 25; 12(5):e0177764. PM ID: 28542388
  • Herrington, KA, et al. (2017) Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol. Biol. Cell. 2017 May 24;. PM ID: 28539409
  • Cruz-Molina, S, et al. (2017) PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation. Cell Stem Cell. 2017 May 4; 20(5):689-705.e9. PM ID: 28285903
  • Wang, L, et al. (2017) Derivation and characterization of primordial germ cells from Guangxi yellow-feather chickens.. Poult. Sci.. 2017 May 1; 96(5):1419-1425. PM ID: 28158811
  • Uehara, T, et al. (2017) Selective degradation of splicing factor CAPER [alpha] by anticancer sulfonamides. Nature Chemical Biology. 2017 Apr 27; 13:675–680. Link: Nature Chemical Biology
  • Qi, Z, et al. (2017) An optimized, broadly applicable piggyBac transposon induction system. Nucleic Acids Res.. 2017 Apr 20; 45(7):e55. PM ID: 28082389
  • Wen, Y, et al. (2017) A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. J. Biol. Chem.. 2017 Apr 14; 292(15):6148-6162. PM ID: 28228480
  • Mitra, A, et al. (2017) IL6-mediated inflammatory loop reprograms normal to epithelial-mesenchymal transition(+) metastatic cancer stem cells in preneoplastic liver of transforming growth factor beta-deficient β2-spectrin(+/-) mice. Hepatology. 2017 Apr 1; 65(4):1222-1236. PM ID: 27863449
  • Zamboni, CG, et al. (2017) Polymeric nanoparticles as cancer-specific DNA delivery vectors to human hepatocellular carcinoma. J Control Release. 2017 Mar 27;. PM ID: 28351668
  • Katayama, H, et al. (2017) Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon. Sci Rep. 2017 Mar 15; 7:44498. PM ID: 28295042
  • Mitzelfelt, KA, et al. (2017) Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection. Stem Cell Reports. 2017 Mar 14; 8:491–499. Link: Stem Cell Reports