Cumate Solution for the PiggyBac Transposon System

Control the cumate-inducible promoter used on SBI’s PiggyBac Vectors with this ready-to-add 10,000x inducer solution

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Cumate Solution, high concentration, 10,000x for use with PiggyBac

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Cumate Solution, high concentration, 10,000x for use with PiggyBac

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500 µL
PBQM100A-1
$ 96
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Overview

The reagent behind tight, titratable gene expression

Prepared specifically for use with SBI’s inducible PiggyBac Vectors, the Cumate Solution for the PiggyBac Transposon System is a ready-to-add 10,000x inducer solution.

With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

How It Works

Tightly-controlled, inducible gene expression

Get robust, titratable gene expression with low background using SBI’s cumate-inducible vectors. These vectors take advantage of CymR, a repressor that binds to cumate operator sequences (CuO) with high affinity in the absence of cumate, a non-toxic small molecule. Providing much lower background expression than similar systems, SBI’s cumate-inducible vectors can provide up to 32-fold induction of gene expression.

How the cumate operator switch works

  • Robust—increase expression up to 32-fold
  • Adjustable—tune expression levels by titrating the amount of cumate
  • Reversible—turn expression on, then off, then on again
  • Powerful—suitable for in vivo applications

Supporting Data

Tight expression control with low background

In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression

Figure 2. In the absence of cumate, the cumate-inducible PiggyBac Vector shows undetectable levels of expression.

The PiggyBac cumate switch is titratable and can be turned off

Figure 3. The PiggyBac cumate switch is titratable and can be turned off.


Citations

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