Easy and consistent shRNA delivery and expression
Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA. The PB-EF1α-GreenPuro-H1-MCS shRNA Cloning and Expression Vector (Cat.# PBSI506A-1) drives production of your shRNA from the strong H1 promoter. The vector also features GFP and puromycin resistance co-expressed from the moderate EF1α promoter, with co-expression mediated by the T2A element.
With the PiggyBac Transposon System, you can:
- Make transgenic cell lines with a single transfection
- Integrate multiple PiggyBac Vectors in a single transfection
- Insert an expression cassette into human, mouse, and rat cells
- Deliver virtually any-sized DNA insert, from 10 – 100 kb
- Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
- Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:
* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.