PB-CMV-miR-302/367-EF1α-GFP iPSC PiggyBac Vector

Take advantage of the easy transgenesis possible with PiggyBac to deliver and constitutively express the miR-302/367 cluster for iPSC generation

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PB-CMV-miR-302/367-EF1α-GFP iPSC Vector

10 µg
PB-MIR302
$ 1610
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Overview

Easy transgenesis means easy reprogramming

Easily and efficiently deliver the miR-302/367 cluster for iPSC generation using the PB-CMV-miR-302/367-EF1α-GFP iPSC PiggyBac Vector. This pre-built vector is ready-to-co-transfect with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1), and expresses miR-302a, miR-302b, miR-302c, miR-302d, and miR-306 with the strong CMV promoter. The vector also includes a GFP reporter to simplify selection of transfectants.

PB-CMV-miR-302/367-EF1α-GFP iPSC PiggyBac Vector

With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Citations

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  • Kirk, JM, et al. (2018) Functional classification of long non-coding RNAs by k-mer content. Nat. Genet.. 2018 Sep 17;. PM ID: 30224646
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  • Strubberg, AM, et al. (2018) The Zinc Finger Transcription Factor PLAGL2 Enhances Stem Cell Fate and Activates Expression of ASCL2 in Intestinal Epithelial Cells. Stem Cell Reports. 2018 Aug 14; 11(2):410-424. PM ID: 30017821
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