PB-CMV-GreenPuro Scramble Hairpin Control Vector

Easily implement a scramble hairpin control for your PiggyBac-delivered shRNA studies with this ready-to-transfect vector

Description
Size
Catalog Number
Price
Quantity
Add to Cart

PB-CMV-GreenPuro Scramble Hairpin Control Vector

Array ( [attributes] => Array ( [attribute_size] => 10 µg ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 563 [display_regular_price] => 563 [image] => Array ( [title] => pb-cmv-greenpuro-scramble-hairpin-control-vector-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01-600x600.png [srcset] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01-600x600.png 600w, https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01-100x100.png 100w [sizes] => (max-width: 600px) 100vw, 600px [full_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01.png [full_src_w] => 2500 [full_src_h] => 2500 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/gene-expression-systems/pb-cmv-greenpuro-scramble-hairpin-control-vector-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 600 [src_h] => 600 ) [image_id] => 11188 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $563 [sku] => PBSI505-000PA-1 [variation_description] =>

PB-CMV-GreenPuro Scramble Hairpin Control Vector

[variation_id] => 64616 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 µg
PBSI505-000PA-1
$ 563
Contact Us Speak to a specialist
1-888-266-5066

Overview

Easy and consistent shRNA delivery and expression

Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA . The PB-CMV-GreenPuro Scramble Hairpin Control Vector (Cat.# PBSI505-000PA-1) is a ready-to-transfect control for your PiggyBac shRNA projects. The vector features GFP and puromycin resistance co-expressed from the strong CMV promoter, with co-expression mediated by the T2A element. An H1 promoter drives expression of the scramble hairpin.

PB-CMV-GreenPuro Scramble Hairpin Control Vector

With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal


Citations

  • Shinmura, K, et al. (2017) WDR62 overexpression is associated with a poor prognosis in patients with lung adenocarcinoma. Mol. Carcinog.. 2017 Aug 1; 56(8):1984-1991. PM ID: 28277612
  • Henssen, AG, et al. (2017) PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat. Genet.. 2017 Jul 1; 49(7):1005-1014. PM ID: 28504702
  • Katayama, M, et al. (2017) Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. J. Reprod. Dev.. 2017 Jun 21; 63(3):311-318. PM ID: 28331164
  • Cammareri, P, et al. (2017) TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis. Cell Death Differ.. 2017 Jun 16;. PM ID: 28622298
  • Kimura, T, et al. (2017) Hsp90 inhibitor geldanamycin attenuates the cytotoxicity of sunitinib in cardiomyocytes via inhibition of the autophagy pathway. Toxicol. Appl. Pharmacol.. 2017 Jun 15; 329:282-292. PM ID: 28624441
  • Maegawa, KI, et al. (2017) The Highly Dynamic Nature of ERdj5 Is Key to Efficient Elimination of Aberrant Protein Oligomers through ER-Associated Degradation. Structure. 2017 Jun 6; 25(6):846-857.e4. PM ID: 28479060
  • Liu, H, et al. (2017) Stochastic Protein Labeling Enables Long-Term Single Molecule Observation in vivo. bioRxiv. 2017 Jun 5;. Link: bioRxiv
  • Lin, W & Li, Z. (2017) Blueberries inhibit cyclooxygenase-1 and cyclooxygenase-2 activity in human epithelial ovarian cancer. Oncol Lett. 2017 Jun 1; 13(6):4897-4904. PM ID: 28599493
  • Park, TS, Kim, SW & Lee, JH. (2017) Efficient transgene expression system using a cumate-inducible promoter and Cre-loxP recombination in avian cells. Asian-australas. J. Anim. Sci.. 2017 Jun 1; 30(6):886-892. PM ID: 27764912
  • Yamazaki, T, et al. (2017) Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase. PLoS ONE. 2017 May 25; 12(5):e0177764. PM ID: 28542388
  • Herrington, KA, et al. (2017) Spatial analysis of Cdc42 activity reveals a role for plasma membrane-associated Cdc42 in centrosome regulation. Mol. Biol. Cell. 2017 May 24;. PM ID: 28539409
  • Cruz-Molina, S, et al. (2017) PRC2 Facilitates the Regulatory Topology Required for Poised Enhancer Function during Pluripotent Stem Cell Differentiation. Cell Stem Cell. 2017 May 4; 20(5):689-705.e9. PM ID: 28285903
  • Wang, L, et al. (2017) Derivation and characterization of primordial germ cells from Guangxi yellow-feather chickens.. Poult. Sci.. 2017 May 1; 96(5):1419-1425. PM ID: 28158811
  • Uehara, T, et al. (2017) Selective degradation of splicing factor CAPER [alpha] by anticancer sulfonamides. Nature Chemical Biology. 2017 Apr 27; 13:675–680. Link: Nature Chemical Biology
  • Qi, Z, et al. (2017) An optimized, broadly applicable piggyBac transposon induction system. Nucleic Acids Res.. 2017 Apr 20; 45(7):e55. PM ID: 28082389
  • Wen, Y, et al. (2017) A stable but reversible integrated surrogate reporter for assaying CRISPR/Cas9-stimulated homology-directed repair. J. Biol. Chem.. 2017 Apr 14; 292(15):6148-6162. PM ID: 28228480
  • Mitra, A, et al. (2017) IL6-mediated inflammatory loop reprograms normal to epithelial-mesenchymal transition(+) metastatic cancer stem cells in preneoplastic liver of transforming growth factor beta-deficient β2-spectrin(+/-) mice. Hepatology. 2017 Apr 1; 65(4):1222-1236. PM ID: 27863449
  • Zamboni, CG, et al. (2017) Polymeric nanoparticles as cancer-specific DNA delivery vectors to human hepatocellular carcinoma. J Control Release. 2017 Mar 27;. PM ID: 28351668
  • Katayama, H, et al. (2017) Generation of non-viral, transgene-free hepatocyte like cells with piggyBac transposon. Sci Rep. 2017 Mar 15; 7:44498. PM ID: 28295042
  • Xu, X, et al. (2017) Reversal of Phenotypic Abnormalities by CRISPR/Cas9-Mediated Gene Correction in Huntington Disease Patient-Derived Induced Pluripotent Stem Cells.. Stem Cell Reports. 2017 Mar 14; 8(3):619-633. PM ID: 28238795