PB-SV40-Neo-EF1α-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector

Take advantage of the large insert capabilities of the PiggyBac Transposon System to simultaneously deliver four mouse reprogramming factors for iPSC generation

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PB-SV40-Neo-EF1α-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector

10 µg
PB400A-1
$ 1059
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Overview

Easy transgenesis means easy reprogramming

With the large insert capabilities of the PiggyBac Transposon System and the system’s easy, consistent transgenesis, you can use a single vector—the PB-SV40-Neo-EF1α-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector—to generate mouse iPSCs. This pre-built vector is ready-to-co-transfect with the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1), and delivers mouse cMyc, Klf4, Oct4, and Sox2 co-expressed from a strong CAGs promoter. The vector also includes a neomycin resistance marker and GFP reporter to simplify selection of transfectants.

PB-SV40-Neo-EF1α-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector

With the PiggyBac Transposon System, you can:

  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)

Customer Agreements
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.
For end user license information, see the following:

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Supporting Data

Easily reprogram mouse cells with the PiggyBac Transposon System

Easily reprogram mouse cells with the PiggyBac Transposon System

Figure 2. Easily reprogram mouse cells with the PiggyBac Transposon System. (A) Gene expression of the PiggyBac Mouse 4-in-1 iPSC Vector was analyzed in 3T3 Cells transfected with either (- lane) the Super PiggyBac Transposase Expression Vector (Cat.# PB210PA-1) alone or (+ lane) the Super PiggyBac Transposase Expression Vector and the PiggyBac Mouse 4-in-1 iPSC Vector. Cells were harvested at two weeks post-transfection, and RNA was isolated and reverse transcribed (RT) into cDNA. Gene expression was evaluated via PCR using primers specific for the four factors and a Gapdh loading control. Only the cells co-transfected with the PiggyBac Mouse 4-in-1 iPSC Vector showed expression of the transcripts. (B). Cells from (A) were also imaged for GFP expression and stained with anti-Sox2 antibody (Texas Red, antibody in the + panel only). DAPI stain was used as a positive control to identify cell nuclei.


Citations

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